产品名称

英文名称：SYBR Green I Gel Staining Solution, 10000x

同义词

Sybr green I、N,N-dimethyl-N'-[4-[(E)-(3-methyl-1,3-benzothiazol-2-ylidene)methyl]-1-phenylquinolin-1-ium-2-yl]-N'-propylpropane-1,3-diamine、SCHEMBL887481、(E)-2-((2-((3-(dimethylamino)propyl)(propyl)amino)-1-phenylquinolin-4(1H)-ylidene)methyl)-3-methylben

产品性质

CAS编号：163795-75-3

分子式：C32H37N4S+

分子量：509.7

PubChem编号：10436340

英文别名：Sybr green I|N,N-dimethyl-N'-[4-[(E)-(3-methyl-1,3-benzothiazol-2-ylidene)methyl]-1-phenylquinolin-1-ium-2-yl]-N'-propylpropane-1,3-diamine|SCHEMBL887481|(E)-2-((2-((3-(dimethylamino)propyl)(propyl)amino)-1-phenylquinolin-4(1H)-ylidene)methyl)-3-methylben

规格或纯度：10000 x in DMSO

英文名称：SYBR Green I Gel Staining Solution, 10000x

储存温度：-20°C储存,充氩

运输条件：超低温冰袋运输

产品介绍：用途SYBR Green I核酸凝胶染液已用于dsDNA定量聚合酶链式反应(PCR)中的DNA染色彗星实验评估精子膜完整性肉眼观察环介导等温扩增反应(LAMP)扩增的DNA作为流式细胞术的荧光染料实时定量逆转录-聚合酶链式反应(qRT-PCR) 的逆转录cDNA染色SYBR Green I stain has been shown to be much less mutagenic than ethidium bromide in Ames tests. SYBR Green I is also a very sensitive stain for oligonucleotides, allowing for detection of as little as 1-2 ng of a synthetic 24-mer on a 5% polyacrylamide gel, which is 50-100 times greater sensitivity than obtained with ethidium bromide. Staining agarose gels with SYBR Green I does not interfere with the transfer of nucleic acids to membranes or subsequent hybridization in Southern or Northern blot analysis as long as 0.1%-0.3% SDS is included in prehybridization and hybridization buffers to remove the dye.

IUPAC Name：N,N-dimethyl-N'-[4-[(E)-(3-methyl-1,3-benzothiazol-2-ylidene)methyl]-1-phenylquinolin-1-ium-2-yl]-N'-propylpropane-1,3-diamine

INCHI：InChI=1S/C32H37N4S/c1-5-20-35(22-13-21-33(2)3)31-23-25(24-32-34(4)29-18-11-12-19-30(29)37-32)27-16-9-10-17-28(27)36(31)26-14-7-6-8-15-26/h6-12,14-19,23-24H,5,13,20-22H2,1-4H3/q+1

InChi Key：CGNLCCVKSWNSDG-UHFFFAOYSA-N

Canonical SMILES：CCCN(CCCN(C)C)C1=[N+](C2=CC=CC=C2C(=C1)C=C3N(C4=CC=CC=C4S3)C)C5=CC=CC=C5

Isomeric SMILES：CCCN(CCCN(C)C)C1=[N+](C2=CC=CC=C2C(=C1)/C=C/3\N(C4=CC=CC=C4S3)C)C5=CC=CC=C5

PubChem CID：10436340

敏感性：对光敏感;对湿度敏感

象形图：

信号词：Warning

危险声明：H341 Suspected of causing genetic defects

预防措施声明：P405,P501,P281,P203,P318

产品包装