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产品详情
产品名称 英文名称:3-Amino-9-ethylcarbazole 同义词 3-AMINO-9-ETHYLCARBAZOLE、132-32-1、9-Ethyl-9H-carbazol-3-amine、3-Amino-N-ethylcarbazole、9H-Carbazol-3-amine, 9-ethyl-、9-Ethylcarbazol-3-amine、Carbazole, 3-amino-9-ethyl-、MFCD00004964、9-ethyl-9H-carbazol-3-ylamine、3-amino-9-ethylcabazole、9-ethyl-3-carbazola 产品性质 CAS编号:132-32-1 分子式:C14H14N2 分子量:210.28 Beilstein号:22(4)4936 MDL号:MFCD00004964 PubChem编号:8588 英文别名:3-AMINO-9-ETHYLCARBAZOLE|132-32-1|9-Ethyl-9H-carbazol-3-amine|3-Amino-N-ethylcarbazole|9H-Carbazol-3-amine, 9-ethyl-|9-Ethylcarbazol-3-amine|Carbazole, 3-amino-9-ethyl-|MFCD00004964|9-ethyl-9H-carbazol-3-ylamine|3-amino-9-ethylcabazole|9-ethyl-3-carbazola 规格或纯度:95% 英文名称:3-Amino-9-ethylcarbazole 生化机理:3-氨基-9-乙基咔唑是一种过氧化酶底物,适用于免疫印迹和免疫组织化学染色程序。该底物能产生一种不溶性红色的末端产物。肉眼或可见光下就可观察。过氧化物酶底物。免疫组化中用于抗体检测的不溶性红色色原,浓度为50X(0.095 mol / l)。 应用:过氧化物酶底物. Usage Prepare samples and incubate with antibodies and HRP-labeled probes, follow with all necessary wash steps. ? Prepare 1X AEC Substrate Buffer (dilute AEC Substrate Buffer, 10X;). ? Dilute one part AEC, 50X with 50 parts 1X AEC Substrate Buffer to make 1X AEC Substrate Solution. Mix well. ? Cover tissue sections with 1X AEC Substrate Solution and incubate 10-30 min. at RT. ? Rinse thoroughly with distilled water. If desired, follow with an appropriate aqueous-based counterstain. ? Mount tissue samples with an aqueous-based mounting medium (e.g., Crystal Mounting Medium; ) ? Unused AEC solution can be discarded according to city, county, state, province or country’s regulations 储存温度:2-8°C储存,充氩 运输条件:冰袋运输 备注:如果有可能,您尽量在使用的当天配置溶液,并在当天使用完它。但是,如果您需要预先配制储备溶液,我们建议您将溶液等份保存在-20°C的密封小瓶中。通常,它们最多可以使用一个月。在使用前和打开样品瓶之前,我们建议您让您的产品在室温下平衡至少1小时。需要更多关于溶解度,用法和处理的建议吗?请访问我们的常见问题(FAQ)页面以获取更多详细信息。 产品介绍:AEC, 50X (3-amino-9-ethylcarbazole); for HRP-based detection systems; provides red chromogen depositions. Precipitable peroxidase substrate for immunohistochemical (IHC) staining techniques. In the presence of horseradish peroxidase (HRP), produces a water-insoluble red oxidation product. Althoμgh not as sensitive as diaminobenzidine (DAB, 50X;, the red deposit formed at the sites of AEC oxidation make this substrate useful for double labeling. Aqueous-based mounting medium and counterstains must be used for IHC applications since oxidized AEC is soluble in alcohols and xylene (e.g., Crystal Mounting Medium; ). Use with AEC Substrate Buffer .过氧化物酶底物. Usage Prepare samples and incubate with antibodies and HRP-labeled probes, follow with all necessary wash steps. ? Prepare 1X AEC Substrate Buffer (dilute AEC Substrate Buffer, 10X;). ? Dilute one part AEC, 50X with 50 parts 1X AEC Substrate Buffer to make 1X AEC Substrate Solution. Mix well. ? Cover tissue sections with 1X AEC Substrate Solution and incubate 10-30 min. at RT. ? Rinse thoroμghly with distilled water. If desired, follow with an appropriate aqueous-based counterstain. ? Mount tissue samples with an aqueous-based mounting medium (e.g., Crystal Mounting Medium; ) ? Unused AEC solution can be discarded according to city, county, state, province or country’s regulationsAEC, 50X (3-amino-9-ethylcarbazole); for HRP-based detection systems; provides red chromogen depositions. Precipitable peroxidase substrate for immunohistochemical (IHC) staining techniques. In the presence of horseradish peroxidase (HRP), produces a water-insoluble red oxidation product. Although not as sensitive as diaminobenzidine (DAB, 50X;, the red deposit formed at the sites of AEC oxidation make this substrate useful for double labeling. Aqueous-based mounting medium and counterstains must be used for IHC applications since oxidized AEC is soluble in alcohols and xylene (e.g., Crystal Mounting Medium; ). Use with AEC Substrate Buffer .Substrate for peroxidase. Usage Prepare samples and incubate with antibodies and HRP-labeled probes, follow with all necessary wash steps. ? Prepare 1X AEC Substrate Buffer (dilute AEC Substrate Buffer, 10X;). ? Dilute one part AEC, 50X with 50 parts 1X AEC Substrate Buffer to make 1X AEC Substrate Solution. Mix well. ? Cover tissue sections with 1X AEC Substrate Solution and incubate 10-30 min. at RT. ? Rinse thoroughly with distilled water. If desired, follow with an appropriate aqueous-based counterstain. ? Mount tissue samples with an aqueous-based mounting medium (e.g., Crystal Mounting Medium; ) ? Unused AEC solution can be discarded according to city, county, state, province or country’s regulations PubChem SID:488180837 PubChem SID url:https//pubchem.ncbi.nlm.nih.gov/substance/488180837 IUPAC Name:9-ethylcarbazol-3-amine INCHI:InChI=1S/C14H14N2/c1-2-16-13-6-4-3-5-11(13)12-9-10(15)7-8-14(12)16/h3-9H,2,15H2,1H3 InChi Key:OXEUETBFKVCRNP-UHFFFAOYSA-N Canonical SMILES:CCN1C2=C(C=C(C=C2)N)C3=CC=CC=C31 Isomeric SMILES:CCN1C2=C(C=C(C=C2)N)C3=CC=CC=C31 RTECS:FE3590000 PubChem CID:8588 UN Number:2811 Packing Group:I Reaxy-Rn:174969 密度:1.17 熔点:98°C-100°C 象形图: 信号词:Danger 危险声明:H350 May cause cancer 预防措施声明:P280,P405,P501,P203,P318 Class:6.1 个人防护装备:Eyeshields, Faceshields, full-face particle respirator type N100 (US), Gloves, respirator cartridge type N100 (US), type P1 (EN143) respirator filter, type P3 (EN 143) respirator cartridges 产品包装
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