产品名称

英文名称：Phosphodiesterase I from Crotalus adamanteus venom

同义词

磷酸二酯酶 、 5'-核酸外切酶 、 寡核苷酸5'-核苷酸水解酶

产品性质

CAS编号：9025-82-5

EC号：232-806-5

MDL号：MFCD00131883

PubChem编号：135277184

酶学委员会编号：3.1.4.1

别名：磷酸二酯酶 | 5'-核酸外切酶 | 寡核苷酸5'-核苷酸水解酶

规格或纯度：≥20 units/mg dry weight

英文名称：Phosphodiesterase I from Crotalus adamanteus venom

单位定义：Unit Definition One Unit hydrolyzes one micromole of p-nitrophenyl thymidine-5-phosphate per minute at 25°C, pH 8.9.

生化机理：Phosphodiesterase I breaks phosphodiester bonds and catalyzes the hydrolysis of various nucleotide polyphosphates. Phosphodiesterase I is released from eucaryotic plasma membranes by phosphatidylinositol-specific phospholipase C.

储存温度：-20°C储存

运输条件：超低温冰袋运输

产品介绍：Venom exonuclease (Phosphodiesterase I) successively hydrolyzes 5'-mononucleotides from 3'-OH-terminated ribo- and deoxyribo-oligonucleotides. The enzyme has an optimal pH range of 9.8-10.4 and a molecular weight of 115 kDa. Phosphodiesterase is inhibited by reducing agents such as glutathione, cysteine and ascorbic acids. It is completely inhibited by 5mM EDTA while ATP, ADP and AMP are partial inhibitors. The enzyme has an absolute requirement for Mg2+.Phosphodiesterase (PDE) is any enzyme that is used to breaks phosphodiester bonds. It is a membrane-bound glycoprotein that is used to catalyze the hydrolysis of various nucleotide polyphosphates. Phosphodiesterase I is used in phosphodiesterase activation assays to hydrolyze AMP.Molecular weight 115,000 (Philipps 1975).Composition Aglycoprotein which binds concanavalin A. (Sulkowski and Laskowski 1961).Optimum pH 9.8-10.4 (Philipps 1975).Inhibitors Reducing agents such as glutathione, cysteine and ascorbic acid (Razell 1963). It is completely inhibited by 5mM EDTA while ATP, ADP and AMP are partial inhibitors.Activators The enzyme has an absolute requirement for Mg2+; Philipps (1975) indicates an optimum concentration of 15 mM.Specificity The enzyme has been purified by Dolapchiev et al. (1974) and Sulkowski and Laskowski, Sr. (1961) with endonuclease activity being eliminated as well as 5'-nucleotidase and nonspecific monophosphatase.It is nonspecific with respect to base or sugar moieties of nucleotides. Avariety of synthetic substrates are hydrolyzed. See review by Razell (1963). Ogilvie and Hruska (1976) indicate that exonuclease will not recognize nucleoside units in the syn conformation. See also Philipps and Chiemprasert (1975).ADP-ribosylated proteins are cleaved at the pyrophosphate linkages by venom phosphodiesterase to yield phosphoribosyl-AMP (Hayaishi 1976).

WGK Germany：1

RTECS：SZ5999500

PubChem CID：135277184

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