产品名称

英文名称：Iodonitrotetrazolium chloride

同义词

Iodonitrotetrazolium chloride、146-68-9、Iodonitrotetrazolium、2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium chloride、Iodonitrotetrazolium violet、INT、Iodonitrotetrazolium purple、Iodonitro tetrazolium、p-Iodonitrotetrazolium Violet、MFCD00011961、XY3JA5、碘硝基氯化四氮唑蓝、2-(对碘苯基)-3-(对硝基苯基)-5-苯基四唑氯水合物、2-(4-碘苯基)-3-(4-硝基苯基)-5-苯基氯化四唑氯水合物、碘硝基氯化四氮唑、INT、对碘硝基四唑紫

产品性质

CAS编号：146-68-9

分子式：C19H13N5O2ICl

分子量：505.71

Beilstein号：4093220

EC号：MFCD00011961

MDL号：MFCD00011961

PubChem编号：64957

别名：碘硝基氯化四氮唑蓝|2-(对碘苯基)-3-(对硝基苯基)-5-苯基四唑氯水合物|2-(4-碘苯基)-3-(4-硝基苯基)-5-苯基氯化四唑氯水合物|碘硝基氯化四氮唑|INT|对碘硝基四唑紫

英文别名：Iodonitrotetrazolium chloride|146-68-9|Iodonitrotetrazolium|2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium chloride|Iodonitrotetrazolium violet|INT|Iodonitrotetrazolium purple|Iodonitro tetrazolium|p-Iodonitrotetrazolium Violet|MFCD00011961|XY3JA5

规格或纯度：98%

英文名称：Iodonitrotetrazolium chloride

生化机理：spectrophotometric assay for succinate dehydrogenase activity Materials spectrophotometer cuvette with 1-cm light patch. Reaction Mixture 100 mmol/liter triethanolamine (HCl), pH8,3 o.5 mmol/liter EDTA, 2 mmol/liter Iodonitrotetrazolium chloride, 12 g/liter Cremophor EL, 20 mmol/liter succinate, pH7.4 Reaction mix 990 ul of reaction mixture with 10ul mitochondrial suspension color develops at 30oC record for 6 min. @500 nm.Tetrazolium dye precursor that forms a purple formazan dye on reduction. Used as an electron acceptor for the colorimetric assay of dehydrogenase activity. Also used for western blot staining and as a staining agent for soft agar colony formation assays.

应用：色度法检测各种脱氢酶的电子受体（Electron acceptor）碘硝基四唑紫已用于细胞染色。它已被用作 D-阿拉伯糖醇的酶促测定中的偶联剂。

储存温度：2-8°C储存

运输条件：冰袋运输

备注：如果有可能，您尽量在使用的当天配置溶液，并在当天使用完它。但是，如果您需要预先配制储备溶液，我们建议您将溶液等份保存在-20°C的密封小瓶中。通常，它们最多可以使用一个月。在使用前和打开样品瓶之前，我们建议您让您的产品在室温下平衡至少1小时。需要更多关于溶解度，用法和处理的建议吗？请访问我们的常见问题（FAQ）页面以获取更多详细信息。

产品介绍：Iodonitrotetrazolium chloride is an electron acceptor used in various colorimetric assays. The chemical has been mainly documented for its measurement of dehydrogenase activity. In the assay to measure SDH (succinate dehydrogenase) activity, Iodonitrotetrazolium chloride was used as an oxidizing agent being reduced by succinate dehydrogenase. Upon reduction the compound forms a formazan dye measured at 500 nm. Iodonitrotetrazolium chloride has also been used to study the electron transport activity in soils, sediments, and pure enzyme cultures as well as nucleotide diphosphate kinase activity.色度法检测各种脱氢酶的电子受体（Electron acceptor）碘硝基四唑紫已用于细胞染色。它已被用作 D-阿拉伯糖醇的酶促测定中的偶联剂。Iodonitrotetrazolium chloride is an electron acceptor used in various colorimetric assays. The chemical has been mainly documented for its measurement of dehydrogenase activity. In the assay to measure SDH (succinate dehydrogenase) activity, Iodonitrotetrazolium chloride was used as an oxidizing agent being reduced by succinate dehydrogenase. Upon reduction the compound forms a formazan dye measured at 500 nm. Iodonitrotetrazolium chloride has also been used to study the electron transport activity in soils, sediments, and pure enzyme cultures as well as nucleotide diphosphate kinase activity.An electron acceptor that forms a formazan dye on reduction; measures dehydrogenase activity.

IUPAC Name：2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazol-3-ium;chloride

INCHI：InChI=1S/C19H13IN5O2.ClH/c20-15-6-8-16(9-7-15)23-21-19(14-4-2-1-3-5-14)22-24(23)17-10-12-18(13-11-17)25(26)27;/h1-13H;1H/q+1;/p-1

InChi Key：JORABGDXCIBAFL-UHFFFAOYSA-M

Canonical SMILES：C1=CC=C(C=C1)C2=NN([N+](=N2)C3=CC=C(C=C3)[N+](=O)[O-])C4=CC=C(C=C4)I.[Cl-]

Isomeric SMILES：C1=CC=C(C=C1)C2=NN([N+](=N2)C3=CC=C(C=C3)[N+](=O)[O-])C4=CC=C(C=C4)I.[Cl-]

WGK Germany：3

PubChem CID：64957

Reaxy-Rn：3846329

溶解性：Soluble in water (4 mg/ml), ethanol (8 mg/ml), ethylene glycol (30 mg/ml), DMSO (slightly), and methanol water (11, 50 mg/ml, very faintly turbid, very deep yellow,hot).

敏感性：易吸潮,对热及光线敏感

熔点：231°C

象形图：

信号词：Warning

危险声明：H315 Causes skin irritationH319 Causes serious eye irritationH335 May cause respiratory irritationH302 Harmful if swallowedH312 Harmful in contact with skinH332 Harmful if inhaled

预防措施声明：P261,P305+P351+P338,P280,P302+P352,P321,P405,P501,P264,P271,P270,P304+P340,P403+P233,P362+P364,P330,P264+P265,P301+P317,P317,P337+P317,P332+P317,P319

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