产品名称

英文名称：Ampliflu Red

同义词

Amplex red 、 1-(3,7-Dihydroxy-10H-phenoxazin-10-yl)ethanone 、 10-Acetyl-3,7-dihydroxyphenoxazine 、 MFCD00467861 、 ADHP 、 Amplex red reagent 、 1-(3,7-dihydroxyphenoxazin-10-yl)ethanone、10-乙酰基-3,7-二羟基吩噁嗪 、 荧光红染料

产品性质

CAS编号：119171-73-2

分子式：C14H11NO4

分子量：257.241

PubChem编号：167453

别名：10-乙酰基-3,7-二羟基吩噁嗪 | 荧光红染料

英文别名：Amplex red | 1-(3,7-Dihydroxy-10H-phenoxazin-10-yl)ethanone | 10-Acetyl-3,7-dihydroxyphenoxazine | MFCD00467861 | ADHP | Amplex red reagent | 1-(3,7-dihydroxyphenoxazin-10-yl)ethanone

规格或纯度：≥98.0% (HPLC),用于荧光分析

英文名称：Ampliflu Red

生化机理：高度敏感，稳定的过氧化物酶荧光底物，用作过氧化氢探针。

储存温度：2-8°C储存,充氩

运输条件：冰袋运输

备注：如果有可能，您尽量在使用的当天配置溶液，并在当天使用完它。但是，如果您需要预先配制储备溶液，我们建议您将溶液等份保存在-20°C的密封小瓶中。通常，它们最多可以使用一个月。在使用前和打开样品瓶之前，我们建议您让您的产品在室温下平衡至少1小时。需要更多关于溶解度，用法和处理的建议吗？请访问我们的常见问题（FAQ）页面以获取更多详细信息。

产品介绍：Fluorogenic substrate for peroxidase;Ampliflu Red is used for the following applications Quantification of inflammatory mediators;Determination of free and esterified cholesterol contents; Evaluation of compounds as substrates for GABA-AT compounds.We are pleased to introduce our serieal Amplite™ fluorogenic substrates to address this unmet need. Our Amplite™ ADHP is a sensitive fluorogenic peroxidase substrate that has much lower background than the material from other commercial vendors. ADHP generates highly fluorescent resorufin that has maximum absorption of 571 nm and maximum emission of 585 nm. Unlike other HRP substrates such as dihydrofluoresceins and dihydrorhodamines, the air-oxidation of ADHP is minimal. So far ADHP has been known as the most sensitive and stable fluorogenic probe for detecting HRP and H2O2. ADHP has been widely used to detect HRP in many immunoassays. On the other hand, ADHP can also be used to detect trace amount of H2O2. The ADHP-based H2O2detection is at least one order of magnitude more sensitive than the commonly used scopoletin assay for H2O2. Because H2O2is produced in many enzymatic redox reactions, ADHP can be used in coupled enzymatic reactions to detect the activity of many oxidases and/orrelated enzymes/substrates or cofactors such as glucose, acetylcholine and cholesterol,L-glutamate, amino acids, etc.Although ADHP exhibits very high sensitivity for detecting peroxidate activity either in a fluorimetric or colorimetric mode, it requires higher pH to reach its maximum fluorescence. We are pleased to introduce our Amplite™ ADAP that has better water solubility and stronger fluorescence than ADHP. Addtionally, our Amplite™is the only fluorogenic peroxidase substrate that generates fluorescent precipitates upon the peroxidase-catalyzed oxidation.Fluorogenic substrate for peroxidase;Ampliflu Red is used for the following applications Quantification of inflammatory mediators;Determination of free and esterified cholesterol contents; Evaluation of compounds as substrates for GABA-AT compounds.We are pleased to introduce our serieal Amplite™ fluorogenic substrates to address this unmet need. Our Amplite™ ADHP is a sensitive fluorogenic peroxidase substrate that has much lower background than the material from other commercial vendors. ADHP generates highly fluorescent resorufin that has maximum absorption of 571 nm and maximum emission of 585 nm. Unlike other HRP substrates such as dihydrofluoresceins and dihydrorhodamines, the air-oxidation of ADHP is minimal. So far ADHP has been known as the most sensitive and stable fluorogenic probe for detecting HRP and H2O2. ADHP has been widely used to detect HRP in many immunoassays. On the other hand, ADHP can also be used to detect trace amount of H2O2. The ADHP-based H2O2detection is at least one order of magnitude more sensitive than the commonly used scopoletinassay for H2O2. Because H2O2is produced in many enzymatic redox reactions, ADHP can be used in coupled enzymatic reactions to detect the activity of many oxidases and/orrelated enzymes/substrates or cofactors such as glucose, acetylcholine and cholesterol,L-glutamate, amino acids, etc.Although ADHP exhibits very high sensitivity for detecting peroxidate activity either in a fluorimetric or colorimetric mode, it requires higher pH to reach its maximum fluorescence. We are pleased to introduce our Amplite™ ADAP that has better water solubility and stronger fluorescence than ADHP. Addtionally, our Amplite™is the only fluorogenic peroxidase substrate that generates fluorescent precipitates upon the peroxidase-catalyzed oxidation.

IUPAC Name：1-(3,7-dihydroxyphenoxazin-10-yl)ethanone

INCHI：InChI=1S/C14H11NO4/c1-8(16)15-11-4-2-9(17)6-13(11)19-14-7-10(18)3-5-12(14)15/h2-7,17-18H,1H3

InChi Key：PKYCWFICOKSIHZ-UHFFFAOYSA-N

Canonical SMILES：CC(=O)N1C2=C(C=C(C=C2)O)OC3=C1C=CC(=C3)O

Isomeric SMILES：CC(=O)N1C2=C(C=C(C=C2)O)OC3=C1C=CC(=C3)O

PubChem CID：167453

敏感性：对光线和空气敏感

熔点：> 250 °C

象形图：

信号词：Warning

危险声明：H302 Harmful if swallowed

预防措施声明：P501,P264,P270,P330,P301+P317

产品包装