产品名称

英文名称：Nuclease S1 from Aspergillus oryzae

同义词

Endonuclease S1、Endonuclease S1

产品性质

CAS编号：37288-25-8

MDL号：MFCD00131010

PubChem编号：135298688

酶学委员会编号：3.1.30.1

别名：Endonuclease S1

英文别名：Endonuclease S1

规格或纯度：≥100,000-500,000 units/ml

英文名称：Nuclease S1 from Aspergillus oryzae

单位定义：One Unit hydrolyzes one microgram of denatured calf thymus DNA per minute at 37°C, pH 4.6.

生化机理：Nuclease S1 isolated from Aspergillus oryzae exhibits endo- and exolytic hydrolytic activity for the phosphodiester bonds of single-stranded DNA and RNA yielding 5’-phosphomononucleotide and 5’-phosphooligonucleotide end-products. It is used to digest non-annealed polynucleotide tails and hairpin loops in RNA and DNA duplexes and can be used to convert superhelical DNA to the linear form.

储存温度：-20°C储存

运输条件：超低温冰袋运输

产品介绍：Product DescriptionChromatographically purified. Specific for single-stranded DNA (ssDNA) degradation. Activity on native (ds) DNA undetectable under the assay conditions. A frozen solution in 30mM sodium acetate, pH 4.6, 50mM NaCl, 1mM ZnCl2, and 50% glycerol.Nuclease S1 isolated from certain Neurospora and Aspergillus species specifically hydrolyzes both terminal and internal phosphodiester bonds of single-stranded DNA and RNA. Nuclease S1 has a molecular weight of approximately 34 kDa and exists as a monomer. The optimum pH range is 4.0-4.6, and it is activated by Zn2+ and/or Ca2+. Inhibitors are EDTA, citrate and high concentrations of SDS.Product ApplicationNuclease S1 from Aspergillus oryzae has been used in a study to assess a biochemical method for mapping mutational alterations in DNA.It has also been used in a study to investigate the DNA damage and repair in a γ-irradiated rat brain tumor.

WGK Germany：2

PubChem CID：135298688

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