产品名称

英文名称：Clostripain from Clostridium histolyticum

同义词

Endoproteinase-Arg-C;Clostridiopeptidase B;Proteinase from Clostridium histolyticum、Endoproteinase-Arg-C;Clostridiopeptidase B;Proteinase from Clostridium histolyticum

产品性质

CAS编号：9028-00-6

EC号：232-822-2

MDL号：MFCD00130814

酶学委员会编号：3.4.22.8

别名：Endoproteinase-Arg-C;Clostridiopeptidase B;Proteinase from Clostridium histolyticum

英文别名：Endoproteinase-Arg-C;Clostridiopeptidase B;Proteinase from Clostridium histolyticum

规格或纯度：≥50 units/mg dry weight

英文名称：Clostripain from Clostridium histolyticum

单位定义：One Unit hydrolyzes one micromole of N-benzoyl-L-arginine ethyl ester per minute at 25°C, pH 7.6, in the presence of dithiothreitol.

生化机理：Clostripain from Clostridium histolyticum is composed of two polypeptide chains, with molecular masses of 41.7 kDa and 15.4 kDa. Clostripain has a highly restricted substrate specificity for Arg-Xaa peptide bonds. Therefore, clostripain has been explored as a potential enzyme for protein sequencing purposes. It has also been studied as a catalyst for condensation of pharmaceutically important peptides containing Arg-Pro bonds.

储存温度：2-8°C储存

运输条件：冰袋运输

产品介绍：Clostripain (Endoproteinase-Arg-C) is a two chain proteinase associated with collagenase and isolated from Clostridium histolyticum. It is highly specific for the carboxyl peptide bond of arginine. Clostripain has a sulfhydryl requirement; it is activated by dithiothreitol, cysteine, or other sulfhydryl containing reagents. The presence of calcium ions is essential. The enzyme is inhibited by oxidizing agents and sulfhydryl reactants and by Co2+, Cu2+, Cd2+, and heavy metal ions. Citrate, borate, and Tris anions are less inhibitory.A protease that cleaves proteins on the carboxyl bond of arginineClostripain (Endoproteinase-Arg-C) is a two chain proteinase associated with collagenase and isolated from Clostridium histolyticum. It is highly specific for the carboxyl peptide bond of arginine. Clostripain has a sulfhydryl requirement; it is activated by dithiothreitol, cysteine, or other sulfhydryl containing reagents. The presence of calcium ions is essential. The enzyme is inhibited by oxidizing agents and sulfhydryl reactants and by Co2+, Cu2+, Cd2+, and heavy metal ions. Citrate, borate, and Tris anions are less inhibitory.A protease that cleaves proteins on the carboxyl bond of arginine

WGK Germany：3

个人防护装备：Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter

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