产品名称

英文名称：Trypsin from bovine pancreas(Purified,Sequencing Grade II)

同义词

Peptidyl peptide hydrolase、Peptidyl peptide hydrolase

产品性质

CAS编号：9002-07-7

EC号：232-650-8

MDL号：MFCD00082094

酶学委员会编号：I.U.B. 3.4.21.4

别名：Peptidyl peptide hydrolase

英文别名：Peptidyl peptide hydrolase

规格或纯度：≥150 units/mg protein (at least 8,625 BAEE/2875 USP/NF units/mg protein)

英文名称：Trypsin from bovine pancreas(Purified,Sequencing Grade II)

单位定义：Unit Definitions TAME Unit One Unit hydrolyzes 1 µmole of p-toluene-sulfonyl-L-arginine methyl ester (TAME) per minute at 25°C, pH 8.2, in the presence of 10 mM calcium.

生化机理：Trypsin cleaves peptide chains, amides and esters mainly at the carboxyl side of the amino acids lysine or arginine. Trypsin originates in the pancreas in the form of the zymogen trypsinogen.

储存温度：-20°C储存

运输条件：超低温冰袋运输

备注：Note 1mg trypsin ≥180 TAME units, 10,350 BAEE units, 3,450 USP/NF units.

产品介绍：Trypsin is a pancreatic serine protease with substrate specificity based upon positively charged lysine and arginine side chains. It is derived from a 34 kDa inactive precursor zymogen, trypsinogen, after enzymatic removal of an N-terminal 6-amino acid leader sequence resulting in the 23.8 kDa trypsin molecule. The optimum pH is 8.0. Trypsin is inhibited by organophosphorus compounds such as diisopropylfluorophosphate and natural inhibitors from pancreas. Soybean, lima bean, and egg white are also sources of natural inhibitors. Trypsin cleaves amide and ester bonds of Arg and Lys. The Aladdin Sequencing Grade Trypsin has been further purified to remove trace contaminating proteases and autolysis products which could interfere in trypsin digestion experiments, and exhibits a single band on PAGE.Trypsin is a serine protease used to hydrolyze proteins. Trypsin from bovine pancreas has a molecular weight of 23.8 kDa. Trypsins are used for the re-suspension of cells during cell culture and in proteomics research for the digestion of various proteinsTrypsin is a pancreatic serine protease with substrate specificity based upon positively charged lysine and arginine side chains. It is derived from a 34 kDa inactive precursor zymogen, trypsinogen, after enzymatic removal of an N-terminal 6-amino acid leader sequence resulting in the 23.8 kDa trypsin molecule. The optimum pH is 8.0. Trypsin is inhibited by organophosphorus compounds such as diisopropylfluorophosphate and natural inhibitors from pancreas. Soybean, lima bean, and egg white are also sources of natural inhibitors. Trypsin cleaves amide and ester bonds of Arg and Lys. The Aladdin Sequencing Grade Trypsin has been further purified to remove trace contaminating proteases and autolysis products which could interfere in trypsin digestion experiments, and exhibits a single band on PAGE.Trypsin is a serine protease used to hydrolyze proteins. Trypsin from bovine pancreas has a molecular weight of 23.8 kDa. Trypsins are used for the re-suspension of cells during cell culture and in proteomics research for the digestion of various proteins

WGK Germany：1

RTECS：YN5075000

敏感性：易潮湿

象形图：

信号词：Danger

危险声明：H315 Causes skin irritationH319 Causes serious eye irritationH335 May cause respiratory irritationH334 May cause allergy or asthma symptoms or breathing difficulties if inhaled

预防措施声明：P261,P342+P311

个人防护装备：dust mask type N95 (US), Eyeshields, Faceshields, Gloves

产品包装