产品名称

英文名称：Trypsin from bovine pancreas(Modified,Sequencing Grade)

同义词

Peptidyl peptide hydrolase、Peptidyl peptide hydrolase

产品性质

CAS编号：9002-07-7

EC号：232-650-8

MDL号：MFCD00082094

别名：Peptidyl peptide hydrolase

英文别名：Peptidyl peptide hydrolase

规格或纯度：≥150 units/mg protein(at least 8,625 BAEE/2875 USP/NF units/mg protein)

英文名称：Trypsin from bovine pancreas(Modified,Sequencing Grade)

单位定义：TAME Unit One Unit hydrolyzes 1 µmole of p-toluene-sulfonyl-L-arginine methyl ester (TAME) per minute at 25°C, pH 8.2, in the presence of 10 mM calcium.

生化机理：Trypsin cleaves peptide chains, amides and esters mainly at the carboxyl side of the amino acids lysine or arginine. Trypsin originates in the pancreas in the form of the zymogen trypsinogen.

储存温度：-20°C储存

运输条件：超低温冰袋运输

备注：Note 1mg trypsin ≥180 TAME units, 10,350 BAEE units, 3,450 USP/NF units.

产品介绍：Trypsin is a pancreatic serine protease with substrate specificity based upon positively charged lysine and arginine side chains. It is derived from a 34 kDa inactive precursor zymogen, trypsinogen, after enzymatic removal of an N-terminal 6-amino acid leader sequence resulting in the 23.8 kDa trypsin molecule. The optimum pH is 8.0. Trypsin is inhibited by organophosphorus compounds such as diisopropylfluorophosphate and natural inhibitors from pancreas. Soybean, lima bean, and egg white are also sources of natural inhibitors. Trypsin cleaves amide and ester bonds of Arg and Lys. The Aladdin Sequencing Grade Trypsin has been further purified to remove trace contaminating proteases and autolysis products which could interfere in trypsin digestion experiments, and exhibits a single band on PAGE.Trypsin is a serine protease used to hydrolyze proteins. Trypsin from bovine pancreas has a molecular weight of 23.8 kDa. Trypsins are used for the re-suspension of cells during cell culture and in proteomics research for the digestion of various proteinsSpecificityTrypsin cleaves peptides on the C-terminal side of lysine and arginine amino acid residues. If aproline residue is on the carboxyl side of the cleavage site, the cleavage will not occur. If an acidic residue is on either side of the cleavage site, the rate of hydrolysis has been shown to be slower.Protein Accession NumberP00760CATH Classification (v. 3.2.0)Class Mainly BetaArchitecture Beta BarrelTopology Thrombin, subunit HMolecular Weight 23.3 kDa (Theoretical)Optimal pH 7.5-8.5 (Koutsopoulos et al. 2007)Isoelectric PointTrypsinogen pH 9.3 (Walsh and Neurath 1964)Trypsin pH 10.5 (Cunningham 1954).Extinction Coefficient Trypsinogen 45,250 cm-1 M-1>Active Site ResiduesHistidine (H63)Aspartic acid (D107)Serine (S200)ActivatorsThe rate of trypsinogen conversion is enhanced by using lanthanide in place of calcium ions (Gomez et al. 1974).InhibitorsPancreatic-, soybean-, lima bean-, and egg white- trypsin inhibitors (see section on Trypsin Inhibitors)DFPAprotininAg+BenzamidineEDTA (White and White 1997)ApplicationsTissue dissociation, especially when combined with other enzymes such as collagenase, and elastaseCell harvesting by “trypsinization”Mitochondria isolationin vitro studies of proteinsRemoving monolayers of cells from plastic and glassVarious hemagglutination proceduresSample preparation for flow cytometric DNA analysisTryptic mappingFingerprinting and sequencing workEnvironmental monitoringReduction of cell density in tissue cultureSubculturing cellsCleavage fusion proteinsGenerating glycopeptides from purified glycoproteins(White and White 1997)

WGK Germany：1

RTECS：YN5075000

敏感性：易潮湿

象形图：

信号词：Danger

危险声明：H315 Causes skin irritationH319 Causes serious eye irritationH335 May cause respiratory irritationH334 May cause allergy or asthma symptoms or breathing difficulties if inhaled

预防措施声明：P261,P342+P311

个人防护装备：dust mask type N95 (US), Eyeshields, Faceshields, Gloves

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