产品名称

英文名称：2-Nitrophenyl β-D-galactopyranoside

同义词

369-07-3、2-Nitrophenyl-beta-D-galactopyranoside、ONPG、2-Nitrophenyl beta-D-galactopyranoside、2-Nitrophenyl beta-D-galactoside、2-Nitrophenyl b-D-galactopyranoside、o-Nitrophenyl beta-D-galactopyranoside、(2R,3R,4S,5R,6S)-2-(hydroxymethyl)-6-(2-nitrophenoxy)te、2-硝基苯基-β-D-吡喃半乳糖苷(ONPG)、邻硝基苯-β-D-吡喃半乳糖苷、o-硝基苯-β-D-半乳糖苷、ONPG

产品性质

CAS编号：369-07-3

分子式：C12H15O8N

分子量：301.25

Beilstein号：92207

EC号：206-716-1

MDL号：MFCD00063255

PubChem编号：96647

别名：2-硝基苯基-β-D-吡喃半乳糖苷(ONPG)|邻硝基苯-β-D-吡喃半乳糖苷|o-硝基苯-β-D-半乳糖苷|ONPG

英文别名：369-07-3|2-Nitrophenyl-beta-D-galactopyranoside|ONPG|2-Nitrophenyl beta-D-galactopyranoside|2-Nitrophenyl beta-D-galactoside|2-Nitrophenyl b-D-galactopyranoside|o-Nitrophenyl beta-D-galactopyranoside|(2R,3R,4S,5R,6S)-2-(hydroxymethyl)-6-(2-nitrophenoxy)te

规格或纯度：99%，非动物源

英文名称：2-Nitrophenyl β-D-galactopyranoside

生化机理：β-galactosidase, breaks down lactose into galactose and glucose. β-Galactosidase is not lactose specific and can act on simple galactosides. 2-Nitrophenyl β-D-galactopyranoside hydrolysis results in the release of galactose and a yellow chromogenic compound. The test substrate does not depend on an induced or constitutive permease enzyme to enter the cell, therefore reactions are rapid and occur within a 24-hour period.β-galactosidase substrate for colorimetric and EIA applications. Hydrolysed by β-galactosidase to yield galactose and ortho-Nitrophenol, which is yellow in color.

应用：用于检测β-半乳糖苷酶(β-galactosidase)活性，是β-半乳糖苷酶的显色底物。经β-半乳糖苷酶催化后可产生黄色产物，在410－420nm波长可以检测吸光度。

储存温度：-20°C储存,充氩

运输条件：超低温冰袋运输

备注：如果有可能，您尽量在使用的当天配置溶液，并在当天使用完它。但是，如果您需要预先配制储备溶液，我们建议您将溶液等份保存在-20°C的密封小瓶中。通常，它们最多可以使用一个月。在使用前和打开样品瓶之前，我们建议您让您的产品在室温下平衡至少1小时。需要更多关于溶解度，用法和处理的建议吗？请访问我们的常见问题（FAQ）页面以获取更多详细信息。

产品介绍：溶于水、甲醇，水中溶解度可达15mg/ml。有吸湿性。 用于检测β-半乳糖苷酶(β-galactosidase)活性，是β-半乳糖苷酶的显色底物。经β-半乳糖苷酶催化后可产生黄色产物，在410－420nm波长可以检测吸光度。Chromogenic substrate for β-galactosidase 2-Nitrophenyl β-D-galactopyranoside is an enzyme substrate used to detect lacZ activity and hence the presence of β-galactosidase.

IUPAC Name：(2R,3R,4S,5R,6S)-2-(hydroxymethyl)-6-(2-nitrophenoxy)oxane-3,4,5-triol

INCHI：InChI=1S/C12H15NO8/c14-5-8-9(15)10(16)11(17)12(21-8)20-7-4-2-1-3-6(7)13(18)19/h1-4,8-12,14-17H,5H2/t8-,9+,10+,11-,12-/m1/s1

InChi Key：KUWPCJHYPSUOFW-YBXAARCKSA-N

Canonical SMILES：C1=CC=C(C(=C1)[N+](=O)[O-])OC2C(C(C(C(O2)CO)O)O)O

Isomeric SMILES：C1=CC=C(C(=C1)[N+](=O)[O-])O[C@H]2[C@@H]([C@H]([C@H]([C@H](O2)CO)O)O)O

WGK Germany：3

PubChem CID：96647

Reaxy-Rn：92207

溶解性：Soluble in water

密度：1.599

敏感性：对光敏感

比旋光度：-68.8 ° (C=1, H2O)

熔点：195°C

个人防护装备：Eyeshields,Gloves,type N95 (US),type P1 (EN143) respirator filter

产品包装