产品名称

英文名称：Endoproteinase Glu-C from Staphylococcus aureus V8

同义词

V8蛋白酶

产品性质

CAS编号：66676-43-5

MDL号：MFCD01324998

酶学委员会编号：3.4.21.19

别名：V8蛋白酶

规格或纯度：≥500 units/mg dry weight

英文名称：Endoproteinase Glu-C from Staphylococcus aureus V8

单位定义：One Unit causes a change of 0.001 A280 nm per minute at 37°C, pH 7.8 using casein as the substrate.

生化机理：Staphylococcus strain V8 protease specifically cleaves peptide bonds on the carboxyl side of aspartic and glutamic acid residues when used in phosphate buffer. When used in ammonium bicarbonate buffer or ammonium acetate buffer cleavage is restricted to the carboxyl side of glutamic acid residues only. The enzyme exhibits maximal activity from pH 4.0 to 7.8. If hemoglobin is used as the substrate, maximal activity is at pH 4.0. The maximal activity is at pH of 7.8 when casein is the substrate.

储存温度：2-8°C储存

运输条件：冰袋运输

产品介绍：Protease S. aureus V8 (Endoproteinase-Glu-C) specifically cleaves peptide bonds on the COOH-terminal side of either aspartic or glutamic acids. In the presence of ammonium, the enzyme specificity is limited to glutamic sites. It has a molecular weight of 27,000 daltons and optimum pH's of 4.0 and 7.8 with hemoglobin as the substrate. Protease S. aureus V8 is inhibited by diisopropylfluorophosphate and monovalent anions such as F-, Cl-, CH3COO- and NO3. Enzyme activity is determined by the casein digestion assay described by Drapeau (Methods Enzymol., 45, 469, 1976).Endoproteinase Glu-C from Staphylococcus aureus strain V8 is a serine protease used for selective cleavage of proteins for amino acid sequence determination or peptide mapping .

WGK Germany：3

象形图：

信号词：Danger

危险声明：H315 Causes skin irritationH319 Causes serious eye irritationH335 May cause respiratory irritationH317 May cause an allergic skin reactionH334 May cause allergy or asthma symptoms or breathing difficulties if inhaled

预防措施声明：P261,P305+P351+P338,P280,P342+P311

个人防护装备：dust mask type N95 (US), Eyeshields, Faceshields, Gloves

产品包装