产品名称

英文名称：Isopropyl beta-D-thiogalactoside

同义词

Isopropyl 1-Thio-β-D-galactopyranoside、异丙基1-硫代-β-D-半乳糖吡喃糖苷

产品性质

CAS编号：367-93-1

分子式：C9H18O5S

分子量：238.3

Beilstein号：4631

EC号：206-703-0

MDL号：MFCD00063273

PubChem编号：656894

别名：异丙基1-硫代-β-D-半乳糖吡喃糖苷

英文别名：Isopropyl 1-Thio-β-D-galactopyranoside

规格或纯度：>98.0%

英文名称：Isopropyl beta-D-thiogalactoside

生化机理：与X-GAL结合使用，用于需要表达β-半乳糖苷酶活性的克隆程序中。半乳糖的不可代谢类似物。

储存温度：2-8°C储存,充氩

运输条件：冰袋运输

产品介绍：IPTG, Dioxane-Free (isopropyl-β-thiogalactoside) is an analogue of allolactose that binds specifically to the repressor protein of the lac operon and induces expression of β-galactosidase in Escherichia coli. The compound is widely used with X-Gal plates to screen for blue and white colonies. The lac operon system and IPTG have been used in multiple cloning experiments and cellular systems to study gene activity.IPTG 常用于需要诱导 β-半乳糖苷酶活性的克隆实验。它常与 X-Gal 或 Bluo-Gal 结合使用，用于重组细菌菌落的蓝白筛选，这些菌落可以诱导 lac 操纵子在大肠杆菌中的表达。IPTG 与 lacI 阻遏蛋白结合并改变其构象而发挥作用，防止 β-半乳糖苷酶编码基因 lacZ 的抑制。IPTG, Dioxane-Free (isopropyl-β-thiogalactoside) is an analogue of allolactose that binds specifically to the repressor protein of the lac operon and induces expression of β-galactosidase in Escherichia coli. The compound is widely used with X-Gal plates to screen for blue and white colonies. The lac operon system and IPTG have been used in multiple cloning experiments and cellular systems to study gene activity.IPTG is commonly used in cloning procedures that require induction of β-galactosidase activity. It is used in conjunction with X-Gal or Bluo-Gal in blue-white selection of recombinant bacterial colonies that induce expression of the lac operon in Escherichia coli. IPTG functions by binding to the lacI repressor and altering its conformation, which prevents the repression of the β-galactosidase coding gene lacZ.

IUPAC Name：(2R,3R,4S,5R,6S)-2-(hydroxymethyl)-6-propan-2-ylsulfanyloxane-3,4,5-triol

INCHI：InChI=1S/C9H18O5S/c1-4(2)15-9-8(13)7(12)6(11)5(3-10)14-9/h4-13H,3H2,1-2H3/t5-,6+,7+,8-,9+/m1/s1

InChi Key：BPHPUYQFMNQIOC-NXRLNHOXSA-N

Canonical SMILES：CC(C)SC1C(C(C(C(O1)CO)O)O)O

Isomeric SMILES：CC(C)S[C@H]1[C@@H]([C@H]([C@H]([C@H](O1)CO)O)O)O

WGK Germany：2

PubChem CID：656894

Reaxy-Rn：4631

溶解性：溶于water, 最高浓度 (mg/mL) 23.83, 最高浓度(mM) 100

密度：1.37

敏感性：对湿度和热敏感

比旋光度：-32° (C=1,H2O)

熔点：116-126°C

象形图：

信号词：Warning

危险声明：H315 Causes skin irritationH319 Causes serious eye irritationH335 May cause respiratory irritationH351 Suspected of causing cancerH302 Harmful if swallowedH312 Harmful in contact with skinH332 Harmful if inhaled

预防措施声明：P261,P305+P351+P338,P280,P302+P352,P321,P405,P501,P264,P271,P270,P304+P340,P403+P233,P362+P364,P330,P203,P264+P265,P301+P317,P318,P317,P337+P317,P332+P317,P319

Merck Index：5082

个人防护装备：Eyeshields,Gloves,half-mask respirator (EU),half-mask respirator (US),multi-purpose combination respirator cartridge (US)

产品包装