产品名称

英文名称：Recombinant Enterokinase

产品性质

CAS编号：9014-74-8

EC号：232-761-1

PubChem编号：168009870

规格或纯度：≥5 units/μl

英文名称：Recombinant Enterokinase

单位定义：1U定义为在25℃，12h~16h之内，将0.5mg保存于25mM Tris-HCl 8.0 缓冲液中的融合蛋白切割95%所需的酶量。

储存温度：-20°C储存

运输条件：超低温冰袋运输

产品介绍：注意事项酶切温度不建议37℃条件下酶切，可能会有非特异性酶切出现。酶切条件在＞200mM 咪唑，或＞200mMNaCl，或＞5%甘油条件下，酶切效果受影响，可参照以下推荐方法进行酶切推荐方法为获得理想酶切结果，请将样品透析到25mM Tris-HCl 8.0缓冲液中，再进行酶切。若不便透析，可将样品稀释到咪唑含量在100mM以下，NaCl浓度在50mM以下，甘油浓度小于5%以下进行酶切，酶的用量与蛋白的比例不变（即1U酶切500µg蛋白）。如果样品溶液中含有上述成分中的一种或多种，且不便去除，此时适当增加酶量或延长酶切时间，也可达到较好酶切效果。磷酸盐的影响磷酸盐对Enterokinase有很强的抑制作用，痕量的磷酸盐都会严重影响Enterokinase的活性，因此在酶切体系内不能存在磷酸盐。去除肠激酶本品是具有高酶活力重组肠激酶，切割蛋白使用量少，可不考虑除去。后续如需去除重组肠激酶，可用阴离子交换树脂（eg.DEAE-FF）对其进行洗脱。推荐洗脱条件如下平衡缓冲液25 mM Tris-HCl pH 8.0洗脱缓冲液25 mM Tris-HCl pH 8.0，含100 mM NaCl酶切效果蛋白酶切效果不好，可适当增加酶量，或适当延长酶切时间。Recombinant Enterokinase is a kind of highly purified recombinant bovine enterokinase. The enzyme has been extensively purified and there are no traces of other contaminating proteases. Enterokinase specifically hydrolyzes peptide bond at the carboxyl side of lysine residue preceded by four aspartic acids Asp-Asp-Asp-Asp-Lys (DDDDK). So, Enterokinase can remove N-terminal fusion protein or tags to get aim protein with native amino acids sequence.Protease that cleaves specifically after a lysine preceded by four aspartic acids Asp-Asp-Asp-Asp-Lys (DDDDK).No any other contaminated proteases, no non-specific cutting sites. One unit is defined as the amount of enzyme needed to cleave 0.5mg of fusion protein in 12 to16 hours to get 95% completion at 25°C in 25mMTris-HCl,pH 8.0. Substrate a special fusion protein.

IUPAC Name：5-benzyl-8,8-dimethylnon-6-ene-1-sulfonic acid;5-[2-(4-tert-butylphenyl)ethenyl]-7-methyloctane-1-sulfonic acid;8,8-dimethyl-5-phenylnon-6-ene-1-sulfonic acid;5-[(4-fluorophenyl)methyl]-8,8-dimethylnon-6-ene-1-sulfonic acid;5-[(4-methoxyphenyl)methyl]-8,8-dimethylnon-6-ene-1-sulfonic acid

INCHI：InChI=1S/C21H34O3S.2C19H30O4S.2C18H27FO3S.2C18H28O3S.C17H26O3S/c1-17(2)16-19(8-6-7-15-25(22,23)24)10-9-18-11-13-20(14-12-18)21(3,4)5;2*1-19(2,3)13-12-16(7-5-6-14-24(20,21)22)15-17-8-10-18(23-4)11-9-17;2*1-18(2,3)12-11-15(6-4-5-13-23(20,21)22)14-16-7-9-17(19)10-8-16;2*1-18(2,3)13-12-17(11-7-8-14-22(19,20)21)15-16-9-5-4-6-10-16;1-17(2,3)13-12-16(15-9-5-4-6-10-15)11-7-8-14-21(18,19)20/h9-14,17,19H,6-8,15-16H2,1-5H3,(H,22,23,24);2*8-13,16H,5-7,14-15H2,1-4H3,(H,20,21,22);2*7-12,15H,4-6,13-14H2,1-3H3,(H,20,21,22);2*4-6,9-10,12-13,17H,7-8,11,14-15H2,1-3H3,(H,19,20,21);4-6,9-10,12-13,16H,7-8,11,14H2,1-3H3,(H,18,19,20)

InChi Key：VRASXGTWKFQRNS-UHFFFAOYSA-N

Canonical SMILES：CC(C)CC(CCCCS(=O)(=O)O)C=CC1=CC=C(C=C1)C(C)(C)C.CC(C)(C)C=CC(CCCCS(=O)(=O)O)CC1=CC=CC=C1.CC(C)(C)C=CC(CCCCS(=O)(=O)O)CC1=CC=CC=C1.CC(C)(C)C=CC(CCCCS(=O)(=O)O)CC1=CC=C(C=C1)OC.CC(C)(C)C=CC(CCCCS(=O)(=O)O)CC1=CC=C(C=C1)OC.CC(C)(C)C=CC(CCCCS(=O)(=O)O)CC1=CC=C(C=C1)F.CC(C)(C)C=CC(CCCCS(=O)(=O)O)CC1=CC=C(C=C1)F.CC(C)(C)C=CC(CCCCS(=O)(=O)O)C1=CC=CC=C1

Isomeric SMILES：CC(C)CC(CCCCS(=O)(=O)O)C=CC1=CC=C(C=C1)C(C)(C)C.CC(C)(C)C=CC(CCCCS(=O)(=O)O)CC1=CC=CC=C1.CC(C)(C)C=CC(CCCCS(=O)(=O)O)CC1=CC=CC=C1.CC(C)(C)C=CC(CCCCS(=O)(=O)O)CC1=CC=C(C=C1)OC.CC(C)(C)C=CC(CCCCS(=O)(=O)O)CC1=CC=C(C=C1)OC.CC(C)(C)C=CC(CCCCS(=O)(=O)O)CC1=CC=C(C=C1)F.CC(C)(C)C=CC(CCCCS(=O)(=O)O)CC1=CC=C(C=C1)F.CC(C)(C)C=CC(CCCCS(=O)(=O)O)C1=CC=CC=C1

关联CAS：9014-74-8

PubChem CID：168009870

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