产品名称

英文名称：5,5＇-Dithio bis-(2-nitrobenzoic acid)

同义词

3,3'-Dithiobis(6-nitrobenzoic acid) 、 DTNB 、 5,5'-Disulfanediylbis(2-nitrobenzoic acid) 、 Ellman's Reagent 、 Dithionitrobenzoic acid 、 3-Carboxy-4-nitrophenyl disulfide 、 5,5'-Dithiobis(2-nitrobenzoic acid) 、 Benzoic acid, 3,3'-dithiobis[6-nitro-Dithiobis、双(3-羧基-4-硝基苯基)二硫 、 二硫代硝基苯甲酸 、 6,6'-二硝基-3,3'-二硫代苯甲酸 、 DTNB, Ellman 试剂

产品性质

CAS编号：69-78-3

分子式：C14H8N2O8S2

分子量：396.35

Beilstein号：1896821

EC号：200-714-4

MDL号：MFCD00007140

PubChem编号：6254

别名：双(3-羧基-4-硝基苯基)二硫 | 二硫代硝基苯甲酸 | 6,6'-二硝基-3,3'-二硫代苯甲酸 | DTNB, Ellman 试剂

英文别名：3,3'-Dithiobis(6-nitrobenzoic acid) | DTNB | 5,5'-Disulfanediylbis(2-nitrobenzoic acid) | Ellman's Reagent | Dithionitrobenzoic acid | 3-Carboxy-4-nitrophenyl disulfide | 5,5'-Dithiobis(2-nitrobenzoic acid) | Benzoic acid, 3,3'-dithiobis[6-nitro-Dithiobis

规格或纯度：98%

英文名称：5,5＇-Dithio bis-(2-nitrobenzoic acid)

储存温度：2-8°C储存,干燥

运输条件：冰袋运输

产品介绍：DTNB (Ellman’s 试剂) 常用于蛋白质和生物分子中巯基 (thiol) 的定量测定。产品应用Ellman′s试剂可用于乙酰胆碱酯酶的检测，并可对半胱氨酸残基进行特异性标记。该试剂是测定蛋白质、多肽和组织中游离巯基含量的敏感试剂。5,5′-二硫代双(2-硝基苯甲酸)是一种水溶性试剂，主要用于巯基的衍生化。Ellman's Reagent (DTNB) reacts with sulfhydryl groups to yield a colored product, providing a reliable method to measure reduced cysteines and other free sulfhydryls in solution. Ellman's Reagent (5,5'-dithio-bis-[2-nitrobenzoic acid]) is used to estimate sulfhydryl groups in a sample by comparing to a standard curve of a sulfhydryl-containing compound such as cysteine. Alternatively, sulfhydryl groups can be assayed using the extinction coefficient of TNB (14,150M-1cm-1 at 412nm). Ellman's Reagent has been used for applications other than sulfhydryl estimation, such as for determining alkylthiols by HPLC using a pre-column derivatization procedure and to study thiols in the active site of several enzymes. Highlights ?Sulfhydryl assay – reacts quantitatively with free (reduced) sulfhydryl groups (–SH) to yield detectable TNB product ?Colorimetric detection – colored product enables spectrophotometric measurement in cuvettes or microplates (λmax = 412nm; ε = 14,150/M?cm) ?Proven method – well-characterized chemistry provides quantitation of peptide or protein sulfhydryl groups using extinction coefficient calculation or by comparison to a cysteine standardA sensitive reagent for measuring the free sulfhydryl content in proteins, peptides, and tissues. DTNB (Ellman’s reagent) is commonly used for quantification of sulfhydryl (thiol) groups in proteins and biomolecules.

IUPAC Name：5-[(3-carboxy-4-nitrophenyl)disulfanyl]-2-nitrobenzoic acid

INCHI：InChI=1S/C14H8N2O8S2/c17-13(18)9-5-7(1-3-11(9)15(21)22)25-26-8-2-4-12(16(23)24)10(6-8)14(19)20/h1-6H,(H,17,18)(H,19,20)

InChi Key：KIUMMUBSPKGMOY-UHFFFAOYSA-N

Canonical SMILES：C1=CC(=C(C=C1SSC2=CC(=C(C=C2)[N+](=O)[O-])C(=O)O)C(=O)O)[N+](=O)[O-]

Isomeric SMILES：C1=CC(=C(C=C1SSC2=CC(=C(C=C2)[N+](=O)[O-])C(=O)O)C(=O)O)[N+](=O)[O-]

WGK Germany：3

RTECS：DG9650000

PubChem CID：6254

Reaxy-Rn：1896821

溶解性：Soluble in 100% ethanol, dioxane, and Phosphate buffer.

密度：1.6412

敏感性：热敏感

熔点：240-245°C

象形图：

信号词：Warning

危险声明：H315 Causes skin irritationH319 Causes serious eye irritationH335 May cause respiratory irritation

预防措施声明：P261,P305+P351+P338,P280,P302+P352,P321,P405,P501,P264,P271,P304+P340,P403+P233,P362+P364,P264+P265,P337+P317,P332+P317,P319

Merck Index：3551

个人防护装备：dust mask type N95 (US),Eyeshields,Gloves

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