产品名称

英文名称：Carbonic Anhydrase from bovine erythrocytes

同义词

Carbonate Dehydratase;Carbonate Hydrolyase、Carbonate Dehydratase;Carbonate Hydrolyase

产品性质

CAS编号：9001-03-0

EC号：232-576-6

MDL号：MFCD00081467

PubChem编号：135330076

酶学委员会编号：4.2.1.1

别名：Carbonate Dehydratase;Carbonate Hydrolyase

英文别名：Carbonate Dehydratase;Carbonate Hydrolyase

规格或纯度：≥3,000 units/mg dry weight

英文名称：Carbonic Anhydrase from bovine erythrocytes

单位定义：One Unit is determined by the electrometric method of Wilbur and Anderson (J. Biol. Chem., 176, 147 (1948)), in which the time required (in seconds) for a saturated CO2 solution to lower the pH of 0.02M Tris-HCl buffer from 8.3 to 6.3, at 0-4°C is determ

生化机理：Carbonic anhydrase is a zinc metalloenzyme that has a molecular weight of approximately 30,000 Da. The enzyme catalyzes the hydration of carbon dioxide to carbonic acid. It is involved in vital physiological and pathological processes such as pH and CO2 homeostasis, transport of bicarbonate and CO2, biosynthetic reactions, bone resorption, calcification, and tumorigenicity. Therefore, this enzyme is an important target for inhibitors with clinical applications for various pathologies such as glaucoma, epilepsy and Parkinson’s disease.

储存温度：2-8°C储存

运输条件：冰袋运输

产品介绍：Carbonic anyhdrase is useful in carboxy group transfers and reduction reactions.Carbonic anhydrase from bovine erythrocytes (BCA) has been used to study the effect of removal of enzyme-bound metal ion, Zn2+, on aggregation behavior of the enzyme. Removal of metal ion by a chelator such as EDTA enhances the propensity of the enzyme to adopt the molten-globule state. This state of the enzyme is found to bind to the chaperone-like α-crystallin and prevent aggregation. The enzyme from Aladdin has been immobilized to electrochemical transducers in order to obtain develop an analytical device for dissolved CO2 measurement. It has also been used for the thermodynamic analysis of conformational changes in BCA.SpecificityBlood CO2 transport and excretion is largely dependent on the rapid catalysis of the CO2 reactions within the erythrocyte by CA (Tufts et al. 2003). Bovine CA reversibly hydrates alkyl pyruvates and it exhibits hydratase activity toward a wide variety of substrates (Pocker et al. 1974, and Wells et al. 1975).Protein Accession NumberP00921CATH Classification (v. 3.2.0)Class Alpha BetaArchitecture RollTopology Carbonic Anhydrase IIMolecular Weight29.0 kDa (Theoretical)30 kDa (Lindskog et al. 1971)Optimal pH7.0-7.5 (Demir et al. 2000, and Tasgin et al. 2009)Isoelectric Point6.40 (Theoretical)Extinction Coefficient50,070 cm-1M-1 (Theoretical)E1%, 280 = 19.0 (Nyman and Lindskog 1964)Active Site ResiduesHistidine (H63)Asparagine (N66)Lysine (K126)ActivatorsHPO42- (Rowlett et al. 1991)SO32- (Rowlett et al. 1991)InhibitorsMonovalent anions (Lindskog et al. 1971, and Ward and Cull 1972)Sulfonates and sulfonamides (Pocker and Watamori 1973, and Binford et al. 1974)Imidazole (Edsall 1968)ApplicationsCO2 determination in bloodElimination of CO2 in reagents for acidity testingCarboxy group transfersReduction reactions

WGK Germany：3

PubChem CID：135330076

个人防护装备：Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter

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