产品名称

英文名称：E-64

同义词

e-64、66701-25-5、E 64、(2S,3S)-3-(((S)-1-((4-Guanidinobutyl)amino)-4-methyl-1-oxopentan-2-yl)carbamoyl)oxirane-2-carboxylic acid、CHEMBL374508、CHEBI30270、E64、R76F7856MV、Thiol protease inhibitor、(2S,3S)-3-[[(2S)-1-[4-(diaminomethylideneamino)butylamino]-4-me、反-环氧丁二酰基-L-亮氨酰胺基(4-胍基)丁烷、L-反-3-羧基环氧乙烷-2-羰基)-L-亮氨酰胍基丁胺、N-(反式-环氧丁二酰基)-L-亮氨酸-胍基丁基酰胺

产品性质

CAS编号：66701-25-5

分子式：C15H27N5O5

分子量：357.41

Beilstein号：1405664

MDL号：MFCD00080261

PubChem编号：123985

别名：反-环氧丁二酰基-L-亮氨酰胺基(4-胍基)丁烷|L-反-3-羧基环氧乙烷-2-羰基)-L-亮氨酰胍基丁胺|N-(反式-环氧丁二酰基)-L-亮氨酸-胍基丁基酰胺

英文别名：e-64|66701-25-5|E 64|(2S,3S)-3-(((S)-1-((4-Guanidinobutyl)amino)-4-methyl-1-oxopentan-2-yl)carbamoyl)oxirane-2-carboxylic acid|CHEMBL374508|CHEBI30270|E64|R76F7856MV|Thiol protease inhibitor|(2S,3S)-3-[[(2S)-1-[4-(diaminomethylideneamino)butylamino]-4-me

规格或纯度：蛋白酶抑制剂,99%

英文名称：E-64

生化机理：E-64是一个不可逆的，强有力的，高选择性半胱氨酸蛋白酶抑制剂。E-64不和非蛋白酶的官能硫基基团发生反应，如L-乳酸脱氢酶或肌酸激酶。 E-64像其他半胱氨酸蛋白酶抑制剂一样，如亮肽素和抗痛素，不会抑制丝氨酸蛋白酶（胰蛋白酶除外）。E-64的反式-环氧丁二酰基（活性部分）不可逆地结合在许多的半胱氨酸蛋白酶的活性巯基基团，如木瓜蛋白酶，猕猴桃酶(actinidase)，组织蛋白酶B,H，和L。 E-64是一种体内研究非常有用的半胱氨酸蛋白酶抑制剂，因为它有专一性的抑制特点，它可渗透到细胞和组织中，并具有低毒性。不可逆的，有效的和选择性的半胱氨酸蛋白酶抑制剂（组织蛋白酶K，S和L在体外的IC 50值分别为1.4、4.1和2.5 nM）。不抑制丝氨酸蛋白酶（胰蛋白酶除外）。

储存温度：避光,-20°C储存,充氩

运输条件：超低温冰袋运输

备注：如果有可能，您尽量在使用的当天配置溶液，并在当天使用完它。但是，如果您需要预先配制储备溶液，我们建议您将溶液等份保存在-20°C的密封小瓶中。通常，它们最多可以使用一个月。在使用前和打开样品瓶之前，我们建议您让您的产品在室温下平衡至少1小时。需要更多关于溶解度，用法和处理的建议吗？请访问我们的常见问题（FAQ）页面以获取更多详细信息。

产品介绍：E-64是一种亲和纯化半胱氨酸蛋白酶的有效配体。当耦合到硫基化的亲和基质时，结合不再是不可逆的，但保留特异性E-64 is a cysteine protease inhibitor that was isolated from the mold Aspergillus japonicus TPR-64. E-64 is also known as N-[N-(L-3-trans-carboxyoxiran-2-carbonyl)-L-leucyl]-agmatine. E-64 effectively inhibits various cysteine proteases, in particular• cathepsin K• cathepsin L• cathepsin SE-64 also acts against other enzymes, such as• calpain• cathepsin B• cathepsin H• papainprotease inhibitor ，E-64 is an effective ligand for affinity purification of cysteine proteases. When coupled to a thiolated affinity matrix, binding is no longer irreversible, but specificity is retainedE-64 is a cysteine protease inhibitor that was isolated from the mold Aspergillus japonicus TPR-64. E-64 is also known as N-[N-(L-3-trans-carboxyoxiran-2-carbonyl)-L-leucyl]-agmatine. E-64 effectively inhibits various cysteine proteases, in particular• cathepsin K• cathepsin L• cathepsin SE-64 also acts against other enzymes, such as• calpain• cathepsin B• cathepsin H• papainapplicationIt is used as a broad spectrum cysteine proteinase and calpain activation inhibitor. Additionally, Prevotella intermediate studies report that E-64 blocks the breakdown of methaemoglobin and the oxidation of oxyhaemoglobin by InpA. E-64 has also been used to study excystation in Giardia lamblia. E-64 is an inhibitor of Calpain, cathepsin K and cathepsin S. E-64 is an effective ligand for affinity purification of cysteine proteases. When coupled to a thiolated affinity matrix, binding is no longer irreversible, but specificity is retained

PubChem SID：488187783

IUPAC Name：(2S,3S)-3-[[(2S)-1-[4-(diaminomethylideneamino)butylamino]-4-methyl-1-oxopentan-2-yl]carbamoyl]oxirane-2-carboxylic acid

INCHI：InChI=1S/C15H27N5O5/c1-8(2)7-9(20-13(22)10-11(25-10)14(23)24)12(21)18-5-3-4-6-19-15(16)17/h8-11H,3-7H2,1-2H3,(H,18,21)(H,20,22)(H,23,24)(H4,16,17,19)/t9-,10-,11-/m0/s1

InChi Key：LTLYEAJONXGNFG-DCAQKATOSA-N

Canonical SMILES：CC(C)CC(C(=O)NCCCCN=C(N)N)NC(=O)C1C(O1)C(=O)O

Isomeric SMILES：CC(C)C[C@@H](C(=O)NCCCCN=C(N)N)NC(=O)[C@@H]1[C@H](O1)C(=O)O

WGK Germany：3

RTECS：RR0390000

PubChem CID：123985

溶解性：Soluble in DMSO (25mg/ml), or water (20mg/ml)

密度：1.44

敏感性：对光线敏感；对湿度敏感

熔点：>170°C

象形图：

信号词：Warning

危险声明：H371 May cause damage to organs

预防措施声明：P405,P501,P264,P260,P270,P308+P316

个人防护装备：Eyeshields,Gloves,type N95 (US),type P1 (EN143) respirator filter

产品包装