产品名称

英文名称：Ribonuclease A from bovine pancreas(DNase & Protease Free)

同义词

9001-99-4、Nuclease, ribo-、L-HISTIDINE, N-beta-ALANYL-、2-(3-aminopropanoylamino)-3-(1H-imidazol-5-yl)propanoic acid、9001-73-4、DL-(3-aminopropanoyl)histidine、108333-82-0、2-(3-aminopropanamido)-3-(1H-imidazol-5-yl)propanoic acid、beta-Alanylhistidine、L-Histid

产品性质

CAS编号：9001-99-4

MDL号：MFCD00132181

PubChem编号：9369

酶学委员会编号：3.1.27.5

英文别名：9001-99-4|Nuclease, ribo-|L-HISTIDINE, N-beta-ALANYL-|2-(3-aminopropanoylamino)-3-(1H-imidazol-5-yl)propanoic acid|9001-73-4|DL-(3-aminopropanoyl)histidine|108333-82-0|2-(3-aminopropanamido)-3-(1H-imidazol-5-yl)propanoic acid|beta-Alanylhistidine|L-Histid

规格或纯度：≥2,000 units/mg protein

英文名称：Ribonuclease A from bovine pancreas(DNase & Protease Free)

单位定义：One Kalnitsky Unit causes an increase in absorbance of 1.0 at 260nm at 37°C and pH 5.0 when yeast ribosomal RNA is hydrolyzed to acid soluble oliognucleotides.

储存温度：-20°C储存

运输条件：超低温冰袋运输

产品介绍：Pancreatic ribonuclease (RNase I) catalyzes cleavage of the phosphodiester bond between the 5'-ribose of a nucleotide and the phosphate group attached to the 3'-ribose of an adjacent pyrimidine nucleotide forming a 2',3'-cyclic phosphate which may then be hydrolyzed to the corresponding 3'-nucleoside phosphate. Ribonuclease A has a molecular weight of 13,700 daltons. It operates in an optimum pH range of 7.0-7.5. Ribonuclease B has a molecular weight of 14,700 ± 300 daltons. It is a glycoprotein which possesses an amino acid composition indistinguishable from that of RNase A. It contains 6 residues of mannose and 2 residues of N-acetylglucosamine per molecule. It is a glycosylated derivative of RNase A. Ribonuclease is inhibited by heavy metal ions and it is competitively inhibited by DNA. Since Molecular Biology Grade RNase A (Product R128619) is essentially free of DNase and protease activities, this product is useful in removing RNA from DNA in nucleic acid work and where other enzymes are used or where intact proteins must be recovered.Ribonuclease A is used to remove RNA from DNA plasmid preparations and protein samples. It is also used in RNA sequence analysis and protection assays.Pancreatic ribonuclease (RNase I) catalyzes cleavage of the phosphodiester bond between the 5'-ribose of a nucleotide and the phosphate group attached to the 3'-ribose of an adjacent pyrimidine nucleotide forming a 2',3'-cyclic phosphate which may then be hydrolyzed to the corresponding 3'-nucleoside phosphate. Ribonuclease A has a molecular weight of 13,700 daltons. It operates in an optimum pH range of 7.0-7.5. Ribonuclease B has a molecular weight of 14,700 ± 300 daltons. It is a glycoprotein which possesses an amino acid composition indistinguishable from that of RNase A. It contains 6 residues of mannose and 2 residues of N-acetylglucosamine per molecule. It is a glycosylated derivative of RNase A. Ribonuclease is inhibited by heavy metal ions and it is competitively inhibited by DNA. Since Molecular Biology Grade RNase A (Product R128619) is essentially free of DNase and protease activities, this product is useful in removing RNA from DNA in nucleic acid work and where other enzymes are used or where intact proteins must be recovered.Ribonuclease A is used to remove RNA from DNA plasmid preparations and protein samples. It is also used in RNA sequence analysis and protection assays.

Isomeric SMILES：C1=C(NC=N1)CC(C(=O)O)NC(=O)CCN

PubChem CID：9369

敏感性：对湿度敏感

象形图：

信号词：Danger

危险声明：H334 May cause allergy or asthma symptoms or breathing difficulties if inhaled

预防措施声明：P261,P501,P284,P304+P340,P342+P316

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