产品名称

英文名称：Ribonuclease A from bovine pancreas

同义词

9001-99-4、Nuclease, ribo-、L-HISTIDINE, N-beta-ALANYL-、2-(3-aminopropanoylamino)-3-(1H-imidazol-5-yl)propanoic acid、9001-73-4、DL-(3-aminopropanoyl)histidine、108333-82-0、2-(3-aminopropanamido)-3-(1H-imidazol-5-yl)propanoic acid、beta-Alanylhistidine、L-Histid、核糖核酸酶 I

产品性质

CAS编号：9001-99-4

MDL号：MFCD00132181

PubChem编号：9369

酶学委员会编号：3.1.27.5

别名：核糖核酸酶 I

英文别名：9001-99-4|Nuclease, ribo-|L-HISTIDINE, N-beta-ALANYL-|2-(3-aminopropanoylamino)-3-(1H-imidazol-5-yl)propanoic acid|9001-73-4|DL-(3-aminopropanoyl)histidine|108333-82-0|2-(3-aminopropanamido)-3-(1H-imidazol-5-yl)propanoic acid|beta-Alanylhistidine|L-Histid

规格或纯度：≥2,500 units/mg dry weight

英文名称：Ribonuclease A from bovine pancreas

单位定义：One Kalnitsky Unit causes an increase in absorbance of 1.0 at 260nm at 37°C and pH 5.0 when yeast ribosomal RNA is hydrolyzed to acid soluble oliognucleotides. One Kunitz unit equals 50 Aladdin units.

生化机理：Ribonucleases do not hydrolyze DNA, because the DNA lacks 2′-OH groups essential for the formation of cyclic intermediates. RNase can hydrolyze RNA from protein samples. Pancreatic RNase A specifically cleaves at the 3′-side of pyrimidine (uracil or cytosine) phosphate bonds.

储存温度：2-8°C储存

运输条件：冰袋运输

产品介绍：核糖核酸酶能专一性地作用于嘧啶碱基，分解RNA从而得到含有3'-UMP，3'-CMP，及其低聚核苷酸。核糖核酸酶是由单链多肽链组成，为分子量约1.4万的球状蛋白质，虽对于稀酸、热、蛋白质分解酶极为稳定，但在碱中不稳定。催化RNA水解的一种核酸内切酶。高纯度核糖核酸酶是从牛胰腺中分离提取的，并进一步用亲和层析的方法制备，以去除残留的其它水解酶的污染。RNase 保护检测 去除非特异结合的RNA 分析RNA 序列 水解蛋白样品中的RNA 纯化DNAA lyophilized preparation equivalent to Code RASE. Contains some aggregates as a result of lyophilization but shows no difference in specific activity.Ribonuclease A is used to remove RNA from DNA plasmid preparations and protein samples. It is also used in RNA sequence analysis and protection assays.Ribonuclease A is used to remove RNA from DNA plasmid preparations and protein samples. Ribonuclease A is used for RNase protection assays, to remove unspecifically bound RNA, analysis of RNA sequences, to hydrolyze RNA contained in protein samples, and the purification of DNA. Ribonuclease A from bovine pancreas has been used in a study to assess the tissue-specific expression of ruminant pancreatic type ribonucleases. Ribonuclease A from bovine pancreas has also been used in a study to investigate pancreas-specific glycosylated protein disulphide-isomerase binding to misfolded proteins and peptides. RNase protection assays Remove unspecifically bound RNA Analysis of RNA sequences Hydrolyze RNA contained in protein samples Purification of DNAproduct descriptionPancreatic ribonuclease (RNase I) catalyzes cleavage of the phosphodiester bond between the 5'-ribose of a nucleotide and the phosphate group attached to the 3'-ribose of an adjacent pyrimidine nucleotide forming a 2',3'-cyclic phosphate which may then be hydrolyzed to the corresponding 3'-nucleoside phosphate. Ribonuclease A has a molecular weight of 13,700 daltons. It operates in an optimum pH range of 7.0-7.5.Ribonuclease B has a molecular weight of 14,700 ± 300 daltons. It is a glycoprotein which possesses an amino acid composition indistinguishable from that of RNase A. It contains 6 residues of mannose and 2 residues of N-acetylglucosamine per molecule. It is a glycosylated derivative of RNase A.Ribonuclease is inhibited by heavy metal ions and it is competitively inhibited by DNA. Since Molecular Biology Grade RNase A (R128619) is essentially free of DNase and protease activities, this product is useful in removing RNA from DNA in nucleic acid work and where other enzymes are used or where intact proteins must be recovered.

Isomeric SMILES：C1=C(NC=N1)CC(C(=O)O)NC(=O)CCN

PubChem CID：9369

敏感性：易潮湿

象形图：

信号词：Danger

危险声明：H334 May cause allergy or asthma symptoms or breathing difficulties if inhaled

预防措施声明：P261,P501,P284,P304+P340,P342+P316

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