产品名称

英文名称：Ribonuclease A from bovine pancreas(Purified)

同义词

RNAsea 、 RNase A、胰核糖核酸酶 、 核糖核酸 3′-嘧啶寡核苷酸水解酶

产品性质

CAS编号：9001-99-4

MDL号：MFCD00132181

PubChem编号：9369

酶学委员会编号：3.1.27.5

别名：胰核糖核酸酶 | 核糖核酸 3′-嘧啶寡核苷酸水解酶

英文别名：RNAsea | RNase A

规格或纯度：≥3,000 units/mg dry weight

英文名称：Ribonuclease A from bovine pancreas(Purified)

单位定义：One Kalnitsky Unit causes an increase in absorbance of 1.0 at 260nm at 37°C and pH 5.0 when yeast ribosomal RNA is hydrolyzed to acid soluble oliognucleotides. One Kunitz unit equals 50 Worthington units.

生化机理：Ribonucleases do not hydrolyze DNA, because the DNA lacks 2′-OH groups essential for the formation of cyclic intermediates. RNase can hydrolyze RNA from protein samples. Pancreatic RNase A specifically cleaves at the 3′-side of pyrimidine (uracil or cytosine) phosphate bonds.

储存温度：2-8°C储存

运输条件：冰袋运输

产品介绍：核糖核酸酶能专一性地作用于嘧啶碱基，分解RNA从而得到含有3'-UMP，3'-CMP，及其低聚核苷酸。核糖核酸酶是由单链多肽链组成，为分子量约1.4万的球状蛋白质，虽对于稀酸、热、蛋白质分解酶极为稳定，但在碱中不稳定。催化RNA水解的一种核酸内切酶。高纯度核糖核酸酶是从牛胰腺中分离提取的，并进一步用亲和层析的方法制备，以去除残留的其它水解酶的污染。RNase 保护检测 去除非特异结合的RNA 分析RNA 序列 水解蛋白样品中的RNA 纯化DNAA lyophilized preparation equivalent to Code RASE. Contains some aggregates as a result of lyophilization but shows no difference in specific activity.Ribonuclease A is used to remove RNA from DNA plasmid preparations and protein samples. It is also used in RNA sequence analysis and protection assays.Ribonuclease A is used to remove RNA from DNA plasmid preparations and protein samples. Ribonuclease A is used for RNase protection assays, to remove unspecifically bound RNA, analysis of RNA sequences, to hydrolyze RNA contained in protein samples, and the purification of DNA. Ribonuclease A from bovine pancreas has been used in a study to assess the tissue-specific expression of ruminant pancreatic type ribonucleases. Ribonuclease A from bovine pancreas has also been used in a study to investigate pancreas-specific glycosylated protein disulphide-isomerase binding to misfolded proteins and peptides. RNase protection assays Remove unspecifically bound RNA Analysis of RNA sequences Hydrolyze RNA contained in protein samples Purification of DNA

Isomeric SMILES：C1=C(NC=N1)CC(C(=O)O)NC(=O)CCN

PubChem CID：9369

敏感性：易潮湿

象形图：

信号词：Danger

危险声明：H334 May cause allergy or asthma symptoms or breathing difficulties if inhaled

预防措施声明：P261,P501,P284,P304+P340,P342+P316

产品包装