产品名称

英文名称：α-Chymotrypsin from bovine pancreas

同义词

Chymotrypsin Substrate I, Colorimetric 、 AKOS024350585 、 4-[[2-[[2-[[1-(4-nitroanilino)-1-oxo-3-phenylpropan-2-yl]amino]-2-oxoethyl]amino]-2-oxoethyl]amino]-4-oxobutanoic acid 、 PD166954 、 CHYMOTRYPSIN 、 DTXSID30988741 、 a-Chymotrypsin 、 FT-0641215、α-胰凝乳蛋白酶

产品性质

CAS编号：9004-07-3

EC号：3.4.21.1

MDL号：MFCD00130481

PubChem编号：4052643

别名：α-胰凝乳蛋白酶

英文别名：Chymotrypsin Substrate I, Colorimetric | AKOS024350585 | 4-[[2-[[2-[[1-(4-nitroanilino)-1-oxo-3-phenylpropan-2-yl]amino]-2-oxoethyl]amino]-2-oxoethyl]amino]-4-oxobutanoic acid | PD166954 | CHYMOTRYPSIN | DTXSID30988741 | a-Chymotrypsin | FT-0641215

规格或纯度：≥35 units/mg protein

英文名称：α-Chymotrypsin from bovine pancreas

单位定义：One Unit hydrolyzes one micromole of benzoyl-L-tyrosine ethyl ester per minute at 25°C, pH 7.8 in the presence of calcium. An activity of 45 units per mg using the above definition, is the equivalent of 10,000 optical density or 1330 N.F. units per mg usi

生化机理：一种丝氨酸蛋白酶，能将与芳香烃或其他大型疏水性侧链（Tyr、Trp、Phe、Met、Leu）结合的肽从键的羧基端水解。 α-Chymotrypsin is a serine peptidase and has 241 amino acid residues contained in three polypeptide chains (A chain-13 residues, B chain-131 residues, and C chain-97 residues) linked by disulfide bridges. Molecular weight of this enzyme is found to be 25 kDa. The pI is 8.75. It selectively hydrolyzes peptide bonds on the C-terminal side of tyrosine, phenylalanine, tryptophan, and leucine. Ca2+ activates and stabilizes the enzyme. The enzyme is inhibited by diisopropyl fluorophosphate (DFP), phenylmethanesulfonyl fluoride (PMSF), N-p-tosyl-L-phenylalanine chloromethyl ketone (TPCK), chymostatin, aprotinin, α1-antitrypsin, and α2-macroglobulin, 10 mM Cu2+ and Hg2+.

储存温度：2-8°C储存

运输条件：冰袋运输

产品介绍：Product DescribtionChymotrypsin preferentially catalyzes the hydrolysis of peptide bonds involving L-isomers of tyrosine, phenylalanine and tryptophan. It also readily acts upon amides and esters of susceptible amino acids. Chymotrypsin catalyzes the hydrolysis of bonds of leucyl, methionyl, asparaginyl and glutamyl residues.Crystallized as zymogen and activated. Dialyzed against 1mM HCl and lyophilized.Crystallized as zymogen and activated. Dialyzed against 1mM HCl and lyophilized.

Isomeric SMILES：C1=CC=C(C=C1)CC(C(=O)NC2=CC=C(C=C2)[N+](=O)[O-])NC(=O)CNC(=O)CNC(=O)CCC(=O)O

WGK Germany：1

RTECS：GC3050000

关联CAS：232-671-2

PubChem CID：4052643

密度：1.047

敏感性：易潮湿

熔点：200℃

象形图：

信号词：Danger

危险声明：H315 Causes skin irritationH319 Causes serious eye irritationH335 May cause respiratory irritationH334 May cause allergy or asthma symptoms or breathing difficulties if inhaled

预防措施声明：P261,P305+P351+P338,P280,P302+P352,P321,P405,P501,P264,P284,P271,P304+P340,P403+P233,P362+P364,P264+P265,P337+P317,P332+P317,P319,P342+P316

个人防护装备：dust mask type N95 (US),Eyeshields,Faceshields,Gloves

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