产品名称

英文名称：X-GlcA

同义词

114162-64-0、X-Gluc (cyclohexanamine)、Cyclohexanamine (2S,3S,4S,5R,6S)-6-((5-bromo-4-chloro-1H-indol-3-yl)oxy)-3,4,5-trihydroxytetrahydro-2H-pyran-2-carboxylate、X-Beta-d-glcua dcha h2o、5-BROMO-4-CHLORO-3-INDOLYL B-D-、5-Bromo-4-chloro-3-indolyl-beta-D-glucu

产品性质

CAS编号：114162-64-0

分子式：C20H26BrClN2O7

分子量：521.8

MDL号：MFCD00058543

PubChem编号：2733578

英文别名：114162-64-0|X-Gluc (cyclohexanamine)|Cyclohexanamine (2S,3S,4S,5R,6S)-6-((5-bromo-4-chloro-1H-indol-3-yl)oxy)-3,4,5-trihydroxytetrahydro-2H-pyran-2-carboxylate|X-Beta-d-glcua dcha h2o|5-BROMO-4-CHLORO-3-INDOLYL B-D-|5-Bromo-4-chloro-3-indolyl-beta-D-glucu

规格或纯度：99%

英文名称：X-GlcA

生化机理：β-葡萄糖醛酸苷酶的发色底物，被该酶转化为深蓝色的酯。用于检测细菌菌落中的Gus基因。

应用：β-葡糖醛酸酶（β-glucuronidase (GUS)）基因检测的显色底物。

储存温度：-20°C储存,充氩

运输条件：超低温冰袋运输

备注：如果有可能，您尽量在使用的当天配置溶液，并在当天使用完它。但是，如果您需要预先配制储备溶液，我们建议您将溶液等份保存在-20°C的密封小瓶中。通常，它们最多可以使用一个月。在使用前和打开样品瓶之前，我们建议您让您的产品在室温下平衡至少1小时。需要更多关于溶解度，用法和处理的建议吗？请访问我们的常见问题（FAQ）页面以获取更多详细信息。

产品介绍：95%的大肠杆菌具有β-葡萄糖苷酶基因，用这种发色底物的培养基可以检测以及定量食品样本如肉、奶制品以及贝类中的大肠杆菌数量，以及在临床上检测尿路感染。国际上普遍采用该产品取代传统方法以精确检测饮用水中的大肠杆菌数量，该方法大大降低了假阳性和假阴性。该产品也用于快速检测植物中GUS基因融合标记。β-葡糖醛酸酶（β-glucuronidase (GUS)）基因检测的显色底物。X-GlcA is a chromogenic substrate for β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. X-GlcA is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype. When X-GlcA is hydrolyzed by β-galactosidase, the resulting product will form a blue precipitate. Lac+ colonies grown in the presence of X-GlcA turn an intense blue color, allowing for easy differentiation between lac+ and lac- colonies.Chromogenic substrate for β-glucuronidase (GUS) gene detection. Suitable for use in selection of recombinant bacterial colonies with the lac+ phenotype.

IUPAC Name：(2S,3S,4S,5R,6S)-6-[(5-bromo-4-chloro-1H-indol-3-yl)oxy]-3,4,5-trihydroxyoxane-2-carboxylic acid;cyclohexanamine

INCHI：InChI=1S/C14H13BrClNO7.C6H13N/c15-4-1-2-5-7(8(4)16)6(3-17-5)23-14-11(20)9(18)10(19)12(24-14)13(21)22;7-6-4-2-1-3-5-6/h1-3,9-12,14,17-20H,(H,21,22);6H,1-5,7H2/t9-,10-,11+,12-,14+;/m0./s1

InChi Key：JXCKZXHCJOVIAV-CYRSAHDMSA-N

Canonical SMILES：C1CCC(CC1)N.C1=CC(=C(C2=C1NC=C2OC3C(C(C(C(O3)C(=O)O)O)O)O)Cl)Br

Isomeric SMILES：C1CCC(CC1)N.C1=CC(=C(C2=C1NC=C2O[C@H]3[C@@H]([C@H]([C@@H]([C@H](O3)C(=O)O)O)O)O)Cl)Br

WGK Germany：3

PubChem CID：2733578

敏感性：对光线，热和空气敏感

象形图：

信号词：Warning

危险声明：H302 Harmful if swallowedH312 Harmful in contact with skinH332 Harmful if inhaled

预防措施声明：P261,P280,P302+P352,P321,P501,P264,P271,P270,P304+P340,P362+P364,P330,P301+P317,P317

个人防护装备：Eyeshields,Gloves,type N95 (US),type P1 (EN143) respirator filter

产品包装