产品名称

英文名称：5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt

同义词

6578-06-9、BCIP、5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt、BCIP p-toluidine salt、X-phosphate p-toluidine salt、p-toluidine 5-bromo-4-chloro-1H-indol-3-yl phosphate、5-Bromo-4-chloro-1H-indol-3-yl phosphate p-toluidine、5-Bromo-4-chloro-3-indolyl ph

产品性质

CAS编号：6578-06-9

分子式：C8H6BrClNO4P·C7H9N

分子量：433.62

EC号：229-506-1

MDL号：MFCD00040636

PubChem编号：81059

英文别名：6578-06-9|BCIP|5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt|BCIP p-toluidine salt|X-phosphate p-toluidine salt|p-toluidine 5-bromo-4-chloro-1H-indol-3-yl phosphate|5-Bromo-4-chloro-1H-indol-3-yl phosphate p-toluidine|5-Bromo-4-chloro-3-indolyl ph

规格或纯度：≥99%(HPLC)

英文名称：5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt

应用：碱性磷酸酶(alkaline phosphatase)的底物。 5-溴-4-氯-3-吲哚磷酸(BCIP) 和 氯化硝基四氮唑蓝(NBT)用于比色法鉴定碱性磷酸酶标记分子. BCIP/NBT 底物系统用途非常广泛包括 Northern Southern, Western blotting, 原位杂交（in situ hybridization）和免疫组织化学。 BCIP 可以有两种盐的形式 二钠盐型溶于水，对甲苯胺型溶于二甲基甲酰胺（DMF）。这些盐型可用于制备和NBT结合的储存溶液和反应缓冲液，构成碱性磷酸酶的底物溶液。 底物系统和碱性磷酸酶一起培养，产生一种不溶的带紫色的NBT双甲腊( diformazan )产物，容易被观察。

储存温度：-20°C储存

运输条件：超低温冰袋运输

产品介绍：For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indoly?l phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry. BCIP is provided in two salt forms the disodium salt which is soluble in water and the p-toluidine form which is soluble in dimethylformamide. These salt forms may be used to prepare a stock solution that in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color. For added convenience, a mixture of BCIP and NBT is provided in an easily dissolvable tablet form or as a ready-to-use liquid.碱性磷酸酶(alkaline phosphatase)的底物。 5-溴-4-氯-3-吲哚磷酸(BCIP) 和 氯化硝基四氮唑蓝(NBT)用于比色法鉴定碱性磷酸酶标记分子. BCIP/NBT 底物系统用途非常广泛包括 Northern Southern, Western blotting, 原位杂交（in situ hybridization）和免疫组织化学。 BCIP 可以有两种盐的形式 二钠盐型溶于水，对甲苯胺型溶于二甲基甲酰胺（DMF）。这些盐型可用于制备和NBT结合的储存溶液和反应缓冲液，构成碱性磷酸酶的底物溶液。 底物系统和碱性磷酸酶一起培养，产生一种不溶的带紫色的NBT双甲腊( diformazan )产物，容易被观察。For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indoly?l phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry. BCIP is provided in two salt forms the disodium salt which is soluble in water and the p-toluidine form which is soluble in dimethylformamide. These salt forms may be used to prepare a stock solution that in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color. For added convenience, a mixture of BCIP and NBT is provided in an easily dissolvable tablet form or as a ready-to-use liquid.Histochemical substrate for alkaline phosphatase.

IUPAC Name：(5-bromo-4-chloro-1H-indol-3-yl) dihydrogen phosphate;4-methylaniline

INCHI：InChI=1S/C8H6BrClNO4P.C7H9N/c9-4-1-2-5-7(8(4)10)6(3-11-5)15-16(12,13)14;1-6-2-4-7(8)5-3-6/h1-3,11H,(H2,12,13,14);2-5H,8H2,1H3

InChi Key：QEIFSLUFHRCVQL-UHFFFAOYSA-N

Canonical SMILES：CC1=CC=C(C=C1)N.C1=CC(=C(C2=C1NC=C2OP(=O)(O)O)Cl)Br

Isomeric SMILES：CC1=CC=C(C=C1)N.C1=CC(=C(C2=C1NC=C2OP(=O)(O)O)Cl)Br

WGK Germany：3

PubChem CID：81059

熔点：196-198°C

象形图：

信号词：Warning

危险声明：H315 Causes skin irritationH319 Causes serious eye irritationH335 May cause respiratory irritationH302 Harmful if swallowedH312 Harmful in contact with skinH332 Harmful if inhaled

预防措施声明：P261,P305+P351+P338,P280,P302+P352,P321,P405,P501,P264,P271,P270,P304+P340,P403+P233,P362+P364,P330,P264+P265,P301+P317,P317,P337+P317,P332+P317,P319

个人防护装备：dust mask type N95 (US),Eyeshields,Gloves

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