产品名称

英文名称：Ovomucoid,Trypsin Inhibitor

同义词

SBTI、SBTI

产品性质

CAS编号：9035-81-8

EC号：232-906-9

MDL号：MFCD00082102

别名：SBTI

英文别名：SBTI

规格或纯度：Animal Free, 来源大豆

英文名称：Ovomucoid,Trypsin Inhibitor

单位定义：The activity of the inhibitors is expressed as the amount of twice crystallized trypsin (Aladdin Item T128774) inhibited per milligram of inhibitor. 1mg trypsin ≥180 TAME units, 10,350 BAEE units, 3,450 USP/NF units.

生化机理：该抑制剂可作用于胰蛋白酶，而对胰凝乳蛋白酶和纤溶酶的作用较弱。 它还可抑制机制与胰蛋白酶、血浆型激肽释放酶和凝血因子X相似的蛋白酶。 胰蛋白酶抑制剂对金属蛋白酶、基于组织型激肽释放酶、酸性蛋白酶或硫代蛋白酶不起作用。 该抑制剂与蛋白酶活性位点形成化学计量比11的复合物，然后在裂解抑制剂上的单个精氨酸-异亮氨酸键，从而发挥作用。 抑制作用可逆且呈pH依赖性。

储存温度：2-8°C储存

运输条件：冰袋运输

产品介绍：易溶于水、生理盐水，不溶于乙醇、丙酮、乙醚。 肽酶抑制药。能抑制胰蛋白酶、糜蛋白酶及纤维蛋白溶解酶。用于纤维蛋白溶解现象、急性胰腺炎及胰腺坏死。Molecular weight21,500 ± 800 (Wu and Scheraga 1972a).Optimum pH7.0.CompositionThe Kunitz soybean inhibitor consists of a single polypeptide chain crosslinked by two disulfide bridges (Steiner 1965). Structural studies of the inhibitor and active site have been reported, Ellis et al. (1975), Woodward and Ellis (1975), Koide et al. (1974), Koide and Ikenaka (1973), Bidlingmeyer et al. (1972), Ikenaka et al. (1971), Papaioannou and Liener (1970), Hixson and Laskowski (1970), Kato and Tominager (1970), and Wu and Scherage (1962). Donovan and Beardslee (1975) have reported on the thermal denaturation of inhibitor complexes.Isoelectric point4.5 (Kunitz 1947).SpecificitySoybean inhibitor inhibits trypsin mole-for-mole and to a lesser extent chymotrypsin. (Bidlingmeyer et al. 1972). Lanchantin et al. (1969) report soybean inhibitor to form a one-to-one complex with beef or human thrombin thus blocking its specific proteolytic capacity to activate prothrombin. Nanninga and Guest (1964) report plasmin to be inhibited. STI has been reported to inhibit leukocytic proteases. (Lieberman and Gawad 1971), but not the esterolytic, proteolytic or elastolytic activities of porcine elastase (Gertler and Feinstein 1971).Soybean Trypsin Inhibitor (Kunitz)Soybean trypsin inhibitor (SBTI) first crystallized by Kunitz (1945) is one of several such inhibitors found in soybeans. (Fratalli 1969; Millar et al. 1969; Fratalli and Steiner 1968; Birk et al. 1967). The best known preparation is that of Kunitz. Steiner and Fratalli (1969) have reviewed the Kunitz and Bowman-Birk inhibitors. A protein (or polypeptide) proteinase inhibitor probably has peptide bonds compatible with the protease reactive site. Finkenstadt and Laskowski (1965 and 1967) and Ozawa and Laskowski (1966) indicate that a single Arg-Ile bond is cleaved by trypsin; a covalent bimolecular complex of inhibitor and trypsin results. On dissociation either virginal or modified inhibitor appears; see Hixson and Laskowski (1970a), Isheda et al. (1970) and Niekamp et al. (1969).Enzyme activity definitionThe activity of the inhibitors is expressed as the amount of twice crystallized trypsin (Aladdin Item T128774) inhibited per milligram of inhibitor. 1mg trypsin ≥180 TAME units, 10,350 BAEE units, 3,450 USP/NF units.

WGK Germany：3

溶解性：balanced salt solution 1 mg/mL;serum-free medium soluble

密度：1.13

象形图：

信号词：Danger

危险声明：H317 May cause an allergic skin reactionH334 May cause allergy or asthma symptoms or breathing difficulties if inhaled

预防措施声明：P261,P280,P342+P311

个人防护装备：Eyeshields,Gloves,type N95 (US),type P1 (EN143) respirator filter

产品包装