产品名称

英文名称：6-Carboxytetramethylrhodamine

同义词

6-Carboxytetramethylrhodamine、91809-67-5、6-TAMRA、4-carboxy-3-[3-(dimethylamino)-6-dimethylazaniumylidenexanthen-9-yl]benzoate、2-(3,6-Bis(dimethylamino)xanthylium-9-yl)-4-carboxybenzoate、9-(2,5-dicarboxyphenyl)-3,6-bis(dimethylamino)-xanthylium, inner salt

产品性质

CAS编号：91809-67-5

分子式：C25H22N2O5

分子量：430.45

PubChem编号：2762604

英文别名：6-Carboxytetramethylrhodamine|91809-67-5|6-TAMRA|4-carboxy-3-[3-(dimethylamino)-6-dimethylazaniumylidenexanthen-9-yl]benzoate|2-(3,6-Bis(dimethylamino)xanthylium-9-yl)-4-carboxybenzoate|9-(2,5-dicarboxyphenyl)-3,6-bis(dimethylamino)-xanthylium, inner salt

规格或纯度：for fluorescence,≥90%

英文名称：6-Carboxytetramethylrhodamine

储存温度：避光,-20°C储存,干燥

运输条件：超低温冰袋运输

产品介绍：6-羧基四甲基罗丹明（6-TAMRA），是用来建立荧光DNA探针和标记蛋白质/肽。相应的琥珀酰亚胺酯是一个单一的异构体荧光团,在DNA自动测序时最为常用。1).SolventsFor the most part, reactive dyes are hydrophobic molecules and should be dissolved in anhydrous dimethylformamide (DMF) or dimethylsulfoxide (DMSO).2).Reaction pHThe labeling reactions of amines with succinimidyl esters are strongly pH dependent. Amine-reactive reagents react with non-protonated aliphatic amine groups, including the terminal amines of proteins and thee-amino groups of lysines. Thus amine acylation reactions are usually carried out above pH 7.5. Protein modifications by succinimidyl esters can typically be done at pH 7.5-8.5, whereas isothiocyanates may require a pH 9.0-10.0 for optimal conjugations.3).Reaction BuffersBuffers that contain free amines such as Tris and glycine and thiol compounds must be avoided when using an amine-reactive reagent. Ammonium salts (such as ammonium sulfate and ammonium acetate) that are widely used for protein precipitation must also be removed (such as viadinlysis) before performing dye conjugations.4).Reaction TemperatureMost conjugations are done at room temperature. However, either elevated or reduced temperature may be required for a particular labeling reaction.6-Carboxytetramethylrhoda?mine (6-TAMRA) is used to create fluorescent DNA probes and labeled proteins/peptides. The corresponding succinimidyl ester is a single isomer fluorophore most commonly used in automated DNA sequencing.1).SolventsFor the most part, reactive dyes are hydrophobic molecules and should be dissolved in anhydrous dimethylformamide (DMF) or dimethylsulfoxide (DMSO).2).Reaction pHThe labeling reactions of amines with succinimidyl esters are strongly pH dependent. Amine-reactive reagents react with non-protonated aliphatic amine groups, including the terminal amines of proteins and thee-amino groups of lysines. Thus amine acylation reactions are usually carried out above pH 7.5. Protein modifications by succinimidyl esters can typically be done at pH 7.5-8.5, whereas isothiocyanates may require a pH 9.0-10.0 for optimal conjugations.3).Reaction BuffersBuffers that contain free amines such as Tris and glycine and thiol compounds must be avoided when using an amine-reactive reagent. Ammonium salts (such as ammonium sulfate and ammonium acetate) that are widely used for protein precipitation must also be removed (such as viadinlysis) before performing dye conjugations.4).Reaction TemperatureMost conjugations are done at room temperature. However, either elevated or reduced temperature may be required for a particular labeling reaction.

IUPAC Name：4-carboxy-3-[3-(dimethylamino)-6-dimethylazaniumylidenexanthen-9-yl]benzoate

INCHI：InChI=1S/C25H22N2O5/c1-26(2)15-6-9-18-21(12-15)32-22-13-16(27(3)4)7-10-19(22)23(18)20-11-14(24(28)29)5-8-17(20)25(30)31/h5-13H,1-4H3,(H-,28,29,30,31)

InChi Key：COCMHKNAGZHBDZ-UHFFFAOYSA-N

Canonical SMILES：CN(C)C1=CC2=C(C=C1)C(=C3C=CC(=[N+](C)C)C=C3O2)C4=C(C=CC(=C4)C(=O)[O-])C(=O)O

Isomeric SMILES：CN(C)C1=CC2=C(C=C1)C(=C3C=CC(=[N+](C)C)C=C3O2)C4=C(C=CC(=C4)C(=O)[O-])C(=O)O

WGK Germany：3

PubChem CID：2762604

溶解性：Soluble in methanol, DMSO, DMF or aqueous buffers (pH > 6.5). , Soluble in methanol, DMSO, DMF or aqueous buffers (pH > 6.5).

密度：1.231

敏感性：对光线敏感，充气保存

象形图：

信号词：Warning

危险声明：H315 Causes skin irritationH319 Causes serious eye irritationH335 May cause respiratory irritation

预防措施声明：P261,P305+P351+P338,P280,P302+P352,P321,P405,P501,P264,P271,P304+P340,P403+P233,P362+P364,P264+P265,P337+P317,P332+P317,P319

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