产品名称

英文名称：Hexokinase from Yeast(Lyophilized)

同义词

己糖激酶

产品性质

CAS编号：9001-51-8

EC号：232-611-5

MDL号：MFCD00131321

PubChem编号：176259338

酶学委员会编号：2.7.1.1

别名：己糖激酶

规格或纯度：≥150 units/mg protein

英文名称：Hexokinase from Yeast(Lyophilized)

单位定义：Unit Definition One Unit reduces one micromole of NAD per minute at 30°C, pH 8.0.

生化机理：Catalyzes the phosphorylation of D-hexose sμgars at the C6 position utilizing ATP as a phosphate source. The rate of phosphorylation varies with different hexoses (pH 7.5, 30 °C)D-fructose KM 0.33 mM;D-glucose KM 0.12 mM;D-mannose KM 0.05 mM. Yeast hexokinase exists as two similar isoforms, PI and PII (A and B), with isoelectric points of 5.25 and 4, respectively.Molecular Weight ~ 54 kDa (monomer);~110 kDa (dimer);Optimal pH 7.5 to 9.0;Extinction Coefficient E1% = 8.85 (PI) and 9.47 (PII) at 280 nm. Activators Hexokinase requires Mg2+ ions (KM = 2.6 mM) for activity. Hexokinase is activated by catecholamines and related compounds. Inhibitors sorbose-1-phosphate, polyphosphates, 6-deoxy-6-fluoroglucose, 2-C-hydroxy-methylglucose?, xylose, lyxose, and thiol reactive compounds (Hg2+ and 4-chloromercuribenzoate).

储存温度：-20°C储存

运输条件：超低温冰袋运输

备注：卖完停产，不再备货

产品介绍：Hexokinase catalyzes the reactionD-hexose + ATP → D-hexose 6-phosphate + ADP Hexokinases have been isolated from the yeast cell in two distinct forms, designated P-I and P-II (Schulze et al. 1969). These are separate, noninterconvertible isozymes (Womack et al. 1973). Hexokinase is used to determine glucose, fructose, mannose and ATP.Catalyzes the phosphorylation of D-hexose sμgars at the C6 position utilizing ATP as a phosphate source. Used to determine glucose, fructose, mannose and ATP.Chromatographically purified. A lyophilized powder containing ~30% buffer/stabilizer. Phosphohexose isomerase, 6-phosphogluconate dehydrogenase, adenylate kinase and creatine phosphokinase contaminant activities are 0.10%, 0.005%, 0.005% and 0.005%, respectively.Hexokinase catalyzes the reactionD-hexose + ATP → D-hexose 6-phosphate + ADP Hexokinases have been isolated from the yeast cell in two distinct forms, designated P-I and P-II (Schulze et al. 1969). These are separate, noninterconvertible isozymes (Womack et al. 1973). Hexokinase is used to determine glucose, fructose, mannose and ATP.Catalyzes the phosphorylation of D-hexose sugars at the C6 position utilizing ATP as a phosphate source. Used to determine glucose, fructose, mannose and ATP.Chromatographically purified. A lyophilized powder containing ~30% buffer/stabilizer. Phosphohexose isomerase, 6-phosphogluconate dehydrogenase, adenylate kinase and creatine phosphokinase contaminant activities are 0.10%, 0.005%, 0.005% and 0.005%, respectively.

PubChem CID：176259338

敏感性：易吸潮

象形图：

信号词：Warning

危险声明：H315 Causes skin irritationH319 Causes serious eye irritation

预防措施声明：P305+P351+P338,P280,P321,P362,P337+P313,P332+P313

产品包装