产品名称

英文名称：JC-1

同义词

jc-1、3520-43-2、47729-63-5、5,5',6,6'-Tetrachloro-1,1',3,3'-tetraethylbenzimidazolocarbocyanine iodide、CBIC2、JC 1、JC1 dye、1,1',3,3'-Tetraethyl-5,5',6,6'-tetrachloroimidacarbocyanine iodide、5,5',6,6'-Tetrachloro-1,1',3,3'-tetraethylbenzimidazolylcarbocyanine、CBIC2、JC1、JC 1、5,5',6,6'-四氯-1,1',3,3'-四乙基-脒酞菁碘化物、5,5',6,6'-四氯-1,1',3,3'-四乙基苯并咪唑碳氰碘化物

产品性质

CAS编号：3520-43-2

分子式：C25H27Cl4IN4

分子量：652.23

MDL号：MFCD00188475

PubChem编号：5492929

别名：CBIC2|JC1|JC 1|5,5',6,6'-四氯-1,1',3,3'-四乙基-脒酞菁碘化物|5,5',6,6'-四氯-1,1',3,3'-四乙基苯并咪唑碳氰碘化物

英文别名：jc-1|3520-43-2|47729-63-5|5,5',6,6'-Tetrachloro-1,1',3,3'-tetraethylbenzimidazolocarbocyanine iodide|CBIC2|JC 1|JC1 dye|1,1',3,3'-Tetraethyl-5,5',6,6'-tetrachloroimidacarbocyanine iodide|5,5',6,6'-Tetrachloro-1,1',3,3'-tetraethylbenzimidazolylcarbocyanine

规格或纯度：95%

英文名称：JC-1

储存温度：避光,-20°C储存,干燥

运输条件：超低温冰袋运输

备注：如果有可能，您尽量在使用的当天配置溶液，并在当天使用完它。但是，如果您需要预先配制储备溶液，我们建议您将溶液等份保存在-20°C的密封小瓶中。通常，它们最多可以使用一个月。在使用前和打开样品瓶之前，我们建议您让您的产品在室温下平衡至少1小时。需要更多关于溶解度，用法和处理的建议吗？请访问我们的常见问题（FAQ）页面以获取更多详细信息。

产品介绍：JC-1 是一种荧光亲脂性羰花青染料，可以用来衡量线粒体的膜电位。双发射电位敏感探针，可用于测量线粒体膜电位。JC-1是一种低膜电位的绿色荧光（λex520 nm）单体。 在较高的电势下，JC-1形成红色荧光（λem596 nm）“ J聚集体”，表现出较宽的激发和非常窄的发射光谱。 JC-1的红色荧光与绿色荧光的比率仅取决于膜电位，不受线粒体大小，形状或密度的影响。线粒体膜电位的下降是细胞凋亡早期的一个标志性事件。通过JC-1从红色荧光到绿色荧光的转变可以很容易地检测到细胞膜电位的下降，同时也可以用JC-1从红色荧光到绿色荧光的转变作为细胞凋亡早期的一个检测指标。JC-1单体的最大激发波长为514nm，最大发射波长为529nm；JC-1聚合物的最大激发波长为585nm，最大发射波长为590nm。实际观察时，使用常规的观察红色荧光和绿色荧光的设置即可。JC-1用于检测细胞的线粒体膜电位时常用的浓度范围为1～20µg/mL，对于很多细胞适宜采用的JC-1浓度为10µg/mL。注意事项JC-1如果一次使用量较小，需把每管再适当进行分装，尽量避免反复冻融。为了您的安全和健康，请穿实验服并戴一次性手套操作。JC-1 is a fluorescent lipophilic carbocyanine dye, which can be used to measure mitochondrial membrane potential.A dual-emission potential-sensitive probe that can be used to measure mitochondrial membrane potential. JC-1 is a green-fluorescent (λex 520 nm) monomer at low membrane potential. At higher potentials, JC-1 forms red-fluorescent (λem 596 nm ) "J-aggregates," which exhibit broad excitation and very narrow emission spectra. The ratio of red to green fluorescence of JC-1 is dependent only on membrane potential, and not influenced by mitochondrial size, shape, or density.The decrease of mitochondrial membrane potential is a landmark event in the early stage of apoptosis. The decrease of cell membrane potential can be easily detected by the transition of JC-1 from red fluorescence to green fluorescence, and the transition from red fluorescence to green fluorescence of JC-1 can also be used as an early detection indicator of apoptosis.The maximum excitation wavelength of JC-1 monomer is 514nm and the maximum emission wavelength is 529nm; the maximum excitation wavelength of JC-1 polymer is 585nm and the maximum emission wavelength is 590nm. For actual observation, use the conventional settings for observing red fluorescence and green fluorescence.The commonly used concentration range of JC-1 for detecting the mitochondrial membrane potential of cells is 1-20μg/mL, and the suitable concentration of JC-1 for many cells is 10μg/mL.PrecautionsIf the amount of JC-1 used at a time is small, each tube needs to be appropriately divided to avoid repeated freeze-thaw.For your safety and health, please wear lab coats and disposable gloves.

IUPAC Name：5,6-dichloro-2-[(E)-3-(5,6-dichloro-1,3-diethylbenzimidazol-3-ium-2-yl)prop-2-enylidene]-1,3-diethylbenzimidazole;iodide

INCHI：InChI=1S/C25H27Cl4N4.HI/c1-5-30-20-12-16(26)17(27)13-21(20)31(6-2)24(30)10-9-11-25-32(7-3)22-14-18(28)19(29)15-23(22)33(25)8-4;/h9-15H,5-8H2,1-4H3;1H/q+1;/p-1

InChi Key：FYNNIUVBDKICAX-UHFFFAOYSA-M

Canonical SMILES：CCN1C2=CC(=C(C=C2[N+](=C1C=CC=C3N(C4=CC(=C(C=C4N3CC)Cl)Cl)CC)CC)Cl)Cl.[I-]

Isomeric SMILES：CCN1C2=CC(=C(C=C2[N+](=C1/C=C/C=C3N(C4=CC(=C(C=C4N3CC)Cl)Cl)CC)CC)Cl)Cl.[I-]

PubChem CID：5492929

溶解性：DMSO

象形图：

信号词：Warning

危险声明：H315 Causes skin irritationH319 Causes serious eye irritationH335 May cause respiratory irritationH302 Harmful if swallowedH410 Very toxic to aquatic life with long lasting effects

预防措施声明：P261,P305+P351+P338,P273,P280,P302+P352,P321,P405,P501,P264,P271,P270,P304+P340,P403+P233,P362+P364,P391,P330,P264+P265,P301+P317,P337+P317,P332+P317,P319

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