4-硝基苯磷酸盐 双环己铵盐

别名

p-Nitrophenyl phosphate bis(cyclohexylammonium) salt,PNPP

溶解性

water: 10 mg/mL, clear, colorless to faintly yellow

应用

4-Nitrophenyl phosphate bis(cyclohexylammonium) salt has been used as a component in the colorimetric reagent for abscisic acid (ABA) assay.

生化/生理作用

p-Nitrophenyl phosphate (pNPP) is the preferred substrate for use with alkaline phosphatase in enzyme-linked immunosorbent assay (ELISA) procedures. Due to its high sensitivity, pNPP is the considered substrate in alkaline phosphatase systems. ELISA applications based on pNPP can be read in timed assays or delayed readings by stopping with alkaline solutions. This substrate yields a yellow-colored soluble end product that can be measured spectrophotometrically at 405 nm.