5-Azido-2-nitrobenzoic acid N-hydroxysuccinimide ester

别名

N-(5-Azido-2-nitrobenzoyloxy)succinimide,N-Succinimidyl 5-azido-2-nitrobenzoate

应用

Photoactive, heterobifunctional cross-linking reagent. Typically, initial reaction couples via ester to primary amine by amide bond formation in the pH range 6.5-8.5. Second bonding occurs during UV irradiation (250-350 nm) via reactive nitrene. The latter bonding is rapid and non-specific.

注意

Reducing agents such as thiols may reduce the azide to amine and should be avoided. Initial manipulations and coupling should be performed under reduced light.