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溴化乙啶
溴化乙啶
中文名称：
溴化乙啶
英文名称：
Ethidium bromide
| CAS： 1239-45-8 | 分子式： | 分子量： | EINECS号： |
Ethidium bromide
基本性质
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折射率
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1.6700 (estimate)
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溶解度
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H2O: 10 mg/mL, opaque, strongly red
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形态
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powder
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颜色
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Red to dark purple
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气味 (Odor)
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Odorless solid
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水溶解性
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40 g/L (25 ºC)
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最大波长(λmax)
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518 nm, 210 nm, 285 nm, 316 nm,
343 nm, 480 nm, 525 nm
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BRN
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3642536
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生物领域应用
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Nucleic acid hybridization; detecting nucleic acids,cells,cancer cells,human cytomegalovirus,hydrogenase A (hydA) of Clostridia,influenza A virus,oligonucleotides,viable Plesiomonas shigelloides; apoptosis assay; nucleic acid quantification
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主要应用
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Electroluminescent displays;photoresists
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EPA化学物质信息
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安全信息
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危险品标志
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安全说明
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危险品运输编号
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UN 2811 6.1/PG 1
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F
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海关编码
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29339990
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毒害物质数据
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毒性
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Intercalating dye widely used to stain
DNA in gels and gradients. The DNA can be visualized readily
by irradiation with ultraviolet light, as little as 0.5 μg of DNA
being detectable by such methods. Ethidium bromide is also
added to cesium chloride density gradients, since as an intercalating
molecule it binds more readily to linear DNA than to
closed collinear circles of DNA (such as plasmids). Binding of
ethidium bromide reduces the density of DNA, thus covalent
circles of DNA have higher densities at saturating concentrations
of ethidium bromide, permitting the separation of plasmid
DNA. Ethidium bromide is a putative carcinogen.
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CHS信息
生产方法及用途
| 用途 |
Ethidium bromide (EtBr) is the most commonly used nucleic acid stain for PAGE or agarose gel electrophoresis. The fluorescence of EtBr increases 21-fold upon binding to double-stranded RNA and 25-fold on binding double-stranded DNA so that destaining the background is not necessary with a low stain concentration. Ethidium bromide has been used in a number of fluorimetric assays for nuc leic acids. It has been shown to bind to single-stranded DNA (although not as strongly) and triple-stranded DNA. Because of its ability to bind to DNA, EtBr is an inhibitor of DNA polymerase. Antiprot ozoal (Trypanosoma). |
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| 用途 |
溴化乙锭是一种高度灵敏的荧光染色剂，用于观察琼脂糖和聚丙烯酰胺凝胶中的DNA。溴化乙锭用标准302nm 紫外光透射仪激发并放射出橙红色信号，可用Polaroid 底片或带CCD 成像头的凝胶成像处理系统拍摄。 对于在电泳后进行凝胶染色，应先用水将储存液样品稀释到 0.5μg/ml，再将凝胶孵育 15-30 分钟。通常无需脱色，但是如果必须降低背景色，则可在水中脱色 15 分钟。然后可在紫外灯箱（254nm 波长）中检测 DNA 条带。也可将溴化乙啶加到凝胶和电泳缓冲液（终浓度 0.5μg/ml）中，并在电泳后立即显影。
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| 用途 |
EB不能穿过活细胞膜,可通过死细胞破损膜对细胞核DNA染色,含一个可嵌入堆积碱基之间的一平面基团,该基团固定位置及与碱基密切接近,致染料与DNA结合现荧光,因EB-DNA复合物荧光产率远大于未结合染料荧光,当电泳凝胶中含游离EB,可测少量DNA,是高灵敏荧光试剂,也是重要荧光染料,常测DNARNA.与DNA结合抑制DNA复制转录
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