磷酸酶(酸性)来源于小麦胚芽

白色冻干粉，溶于水，最适pH值小于7.0，有三个同工酶E_I、E_II、E_III，最适pH值分别为5.5、4.5、4.0；对焦磷酸盐有最高活性。稳定性：酶液于10℃甲苯存在下可保存5个月活力不变。抑制剂有正磷酸盐、钼酸盐和氟化物。能促进血中磷酸酯游离出无机磷酸盐，酶反应：正磷酸单酯+H2O═醇+正磷酸盐。

以土豆为原料，榨汁后分别用丙酮、硫酸铵分级沉淀，酸处理、氧化铝 Cr吸附、离心，最后硫酸铵盐析得产品。

用于生化研究，临床上用作诊断酶，诊断前列腺癌。

-20°C储存

Acid phosphatase is an esterase with broad activity at an optimal pH below 7.0. There are three isozymes, EI, EII, and EIII of similar molecular weight (55 kDa± 5 kDa). Their optimum pH's are 5.5, 4.5, and 4.0 respectively. Acid phosphatase activity was observed by Teller Aladdin Library Archives in 1954 in preparations of a wheat germ lipase described by Singer JBC, 174, 11, in 1948. Equivalent commercial preparations have been distributed labeled as lipase and acid phosphatase thus generating some confusion. Subsequent work has confirmed that the non-specific esterase activity of the wheat germ preparation may be measured both as lipase (triacetin as substrate) and phosphatase. The enzyme assay is based on the work of Brandenberger and Hanson (Helv. Chim. Acta, 36, 900, 1953) and Hofstee ( Arch. Biochem. Biophys., 51, 239, 1954).Acid phosphatase (APase) non-specifically catalyzes the hydrolysis of monoesters and anhydrides of phosphoric acid to produce inorganic phosphate. It is used to study the production, transport, and recycling of phosphate and the metabolic and energy transduction processes of the cell.Acid phosphatase is an esterase with broad activity at an optimal pH below 7.0. There are three isozymes, EI, EII, and EIII of similar molecular weight (55 kDa± 5 kDa). Their optimum pH's are 5.5, 4.5, and 4.0 respectively. Acid phosphatase activity was observed by Teller Aladdin Library Archives in 1954 in preparations of a wheat germ lipase described by Singer JBC, 174, 11, in 1948. Equivalent commercial preparations have been distributed labeled as lipase and acid phosphatase thus generating some confusion. Subsequent work has confirmed that the non-specific esterase activity of the wheat germ preparation may be measured both as lipase (triacetin as substrate) and phosphatase. The enzyme assay is based on the work of Brandenberger and Hanson (Helv. Chim. Acta, 36, 900, 1953) and Hofstee ( Arch. Biochem. Biophys., 51, 239, 1954).Acid phosphatase (APase) non-specifically catalyzes the hydrolysis of monoesters and anhydrides of phosphoric acid to produce inorganic phosphate. It is used to study the production, transport, and recycling of phosphate and the metabolic and energy transduction processes of the cell.Characteristics of Acid Phosphatase from Wheat GermMolecular weight55,000 ± 5,000 (Verjee 1969).CompositionThree isozymes of closely similar molecular weights have been reported by Verjee (1969) EI, EII, and EIII. See also Brouillard and Ouellet (1965).Optimal pHEI - 5.5, EII - 4.5, and EIII - 4.0. (Verjee 1969).SpecificityThe enzyme has a broad esterase activity. See Joyce and Grisolia (1960). It shows highest activity for pyrophosphate.InhibitorsFluoride, molybdate and orthophosphate (Verjee 1969).